Intramolecular carbon isotope distribution of acetic acid in vinegar.
Intramolecular carbon isotope distribution of acetic acid in vinegar.
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DOI：
10.1021/jf200227e
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发表时间：
2011-08
影响因子：
6.1
通讯作者：
Ryota Hattori;Keita Yamada;M. Kikuchi;Satoshi Hirano;N. Yoshida
中科院分区：
文献类型：
--
作者：
Ryota Hattori;Keita Yamada;M. Kikuchi;Satoshi Hirano;N. Yoshida
醋酸碳同位素分析可用于食醋的产地鉴别和质量控制。可能需要分子内碳同位素分布，即乙酸分子中甲基和羧基碳的碳同位素比，以获得更详细的信息来区分这种来源。采用改进的气相色谱-热解-气相色谱-燃烧-同位素比值质谱（GC-Py-GC-C-IRMS）结合顶空固相微萃取（HS-SPME）技术，测定了14种日本醋中乙酸的分子内碳同位素分布。结果表明，从植物生产的醋中的乙酸分子的甲基碳主要是同位素亏损的（13）C相对于羧基碳。此外，同位素差异（δ（13）C（羧基）- δ（13）C（甲基））有很大的范围从-0.3到18.2‰，这些值之间的植物起源，C3，C4，CAM植物不同。
Compound-specific carbon isotope analysis of acetic acid is useful for origin discrimination and quality control of vinegar. Intramolecular carbon isotope distributions, which are each carbon isotope ratios of the methyl and carboxyl carbons in the acetic acid molecule, may be required to obtain more detailed information to discriminate such origin. In this study, improved gas chromatography-pyrolysis-gas chromatography-combustion-isotope ratio mass spectrometry (GC-Py-GC-C-IRMS) combined with headspace solid-phase microextraction (HS-SPME) was used to measure the intramolecular carbon isotope distributions of acetic acid in 14 Japanese vinegars. The results demonstrated that the methyl carbons of acetic acid molecules in vinegars produced from plants were mostly isotopically depleted in (13)C relative to the carboxyl carbon. Moreover, isotopic differences (δ(13)C(carboxyl) - δ(13)C(methyl)) had a wide range from -0.3 to 18.2‰, and these values differed among botanical origins, C3, C4, and CAM plants.