Evidence for dimerization of dimers in K+ channel assembly.
Evidence for dimerization of dimers in K+ channel assembly.
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DOI：
10.1016/s0006-3495(99)77358-3
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发表时间：
1999-04
影响因子：
3.4
通讯作者：
L. Tu;Carol Deutsch
中科院分区：
文献类型：
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作者：
L. Tu;Carol Deutsch
电压门控钾离子通道是四聚体，但四个亚基如何组装尚不清楚。我们分析了失活动力学和峰值电流水平引起的各种野生型和突变Kv1.3亚基，单独表达，组合，并作为串联结构，以显示1）主导途径涉及二聚体的二聚化，和2）二聚体-二聚体相互作用可能涉及的相互作用位点，不同于那些参与单体-单体缔合。此外，使用非变性凝胶电泳，我们检测到的二聚体和四聚体，但不是三聚体，在翻译反应的Kv1.3单体。
Voltage-gated K+channels are tetrameric, but how the four subunits assemble is not known. We analyzed inactivation kinetics and peak current levels elicited for a variety of wild-type and mutant Kv1.3 subunits, expressed singly, in combination, and as tandem constructs, to show that 1) the dominant pathway involves a dimerization of dimers, and 2) dimer-dimer interaction may involve interaction sites that differ from those involved in monomer-monomer association. Moreover, using nondenaturing gel electrophoresis, we detected dimers and tetramers, but not trimers, in the translation reaction of Kv1.3 monomers.