Deficiency of the tryptase-positive, chymase-negative mast cell type in gastrointestinal mucosa of patients with defective T lymphocyte function.
Deficiency of the tryptase-positive, chymase-negative mast cell type in gastrointestinal mucosa of patients with defective T lymphocyte function.
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DOI：
10.4049/jimmunol.138.12.4381
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发表时间：
1987-06
影响因子：
4.4
通讯作者：
A. Irani;S. Craig;G. Deblois;C. Elson;N. Schechter;L. Schwartz
中科院分区：
文献类型：
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作者：
A. Irani;S. Craig;G. Deblois;C. Elson;N. Schechter;L. Schwartz
在正常人、炎症性肠病患者和免疫缺陷疾病患者的胃肠道Carnoy固定标本中，检测了人T （trypase -阳性，乳糜酶-阴性）和TC （trypase -阳性，乳糜酶-阳性）肥大细胞的分布和浓度。在正常标本中，T肥大细胞以粘膜为主（89%），平均浓度为17,850 +/- 4,998 / mm3 (+/- SD, n = 16)，而TC肥大细胞以粘膜下层为主（90%），平均浓度为7,516 +/- 1,227 / mm3 （+/- SD, n = 16）。5例活动期克罗恩病患者回肠标本和3例活动期溃疡性结肠炎患者结肠标本中T和TC肥大细胞浓度与正常值无显著差异（p > 0.4）。三名合并免疫缺陷疾病的患者表现出肠黏膜T肥大细胞浓度显著下降，至540 +/- 630，T肥大细胞百分比相应下降至9%。粘膜和粘膜下层TC肥大细胞的浓度没有变化。在3例获得性免疫缺陷综合征患者中，在回肠粘膜中观察到类似的T肥大细胞类型缺乏，平均浓度为788 +/- 534 T肥大细胞/ mm3，但在3例患者中的1例阑尾和结肠粘膜中没有。这些发现表明功能性T淋巴细胞在人类T肥大细胞类型发育中的作用，并提示T和TC肥大细胞发育的不同途径。
The distribution and concentration of human T (tryptase-positive, chymase-negative) and TC (tryptase-positive, chymase-positive) mast cells were examined in Carnoy's-fixed specimens of the gastrointestinal tract of normal individuals, patients with inflammatory bowel diseases, and patients with immunodeficiency disorders. In normal specimens, T mast cells predominated in the mucosa (89%), with a mean concentration of 17,850 +/- 4,998 per mm3 (+/- SD, n = 16), whereas TC mast cells predominated in the submucosa (90%) with a mean concentration of 7,516 +/- 1,227 per mm3 (+/- SD, n = 16). The concentrations of T and TC mast cells in specimens of ileum from five patients with active Crohn's disease and of colon from three patients with active ulcerative colitis were not significantly different (p greater than 0.4) from normal values. Three patients with combined immunodeficiency disorders demonstrated a marked decrease in the concentration of the T mast cells in the intestinal mucosa, to 540 +/- 630, and a corresponding decrease in the percentage of T mast cells to 9%. Concentrations of TC mast cells were unchanged, both in the mucosa and in the submucosa. In three patients with acquired immunodeficiency syndrome, a similar deficiency of the T mast cell type was observed in the ileal mucosa, with a mean concentration of 788 +/- 534 T mast cells per mm3, but not in the appendiceal and colonic mucosa of one of the three patients. These findings indicate a role for functional T lymphocytes in the development of the T mast cell type in humans, and suggest divergent pathways for development of T and TC mast cells.