ROLE OF LAMININ IN ENDOTHELIAL-CELL RECOGNITION AND DIFFERENTIATION
ROLE OF LAMININ IN ENDOTHELIAL-CELL RECOGNITION AND DIFFERENTIATION
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DOI：
10.1038/ki.1993.5
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发表时间：
1993-01-01
影响因子：
19.6
通讯作者：
GRANT, DS
中科院分区：
文献类型：
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作者：
SCHNAPER, HW;KLEINMAN, HK;GRANT, DS
血管内皮在正常情况下处于静止状态，但在一定条件下会发生明显的行为变化，形成新的血管结构。各种生长和分化因子和细胞外环境之间的复杂相互作用调节着这种行为。影响内皮细胞行为的一系列信号是由基膜的主要结构蛋白层粘连蛋白提供的。在内皮细胞分化的体外实验中，这些信号已经用matrix（一种从小鼠Englebreth-Holm-Swarm肉瘤中重建的基底膜制备物）进行了研究。层粘连蛋白分子的三个生物活性序列已被评估。合成肽包括- rgd -， - yigsr -和- sikvav -序列，分别介导细胞与Matrigel的结合，细胞形态的改变，以及诱导迁移和胶原酶活性。初步数据表明，该系统的观察结果可能与体内内皮功能有关。因此，内皮细胞在基质上的分化可能是一个有用的体外模型，用于研究血管生成的某些步骤。
The vascular endothelium normally is maintained in a quiescent state, but under certain conditions it is induced to undergo marked changes in behavior and form new vascular structures. A complex interaction among various growth and differentiation factors and the extracellular milieu regulates this behavior. One series of signals affecting endothelial behavior is provided by laminin, a major structural protein of basement membrane. These signals have been studied using Matrigel, a reconstituted basement membrane preparation from the murine Englebreth-Holm-Swarm sarcoma, in an in vitro assay of endothelial cell differentiation. Three biologically-active sequences from the laminin molecule have been evaluated. Synthetic peptides that include the sequences -RGD-, -YIGSR-, and -SIKVAV- mediate, respectively, cell binding to Matrigel, alterations in cell morphology, and induction of migration and collagenase activity. Preliminary data indicate that observations made with this system may be relevant to endothelial function in vivo. Endothelial cell differentiation on Matrigel may thus be a useful in vitro model for the study of certain steps in angiogenesis.