Macrophage infiltration into experimental brain metastases: occurrence through an intact blood-brain barrier.
Macrophage infiltration into experimental brain metastases: occurrence through an intact blood-brain barrier.
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巨噬细胞浸润实验性脑转移：通过完整的血脑屏障发生。
DOI：
10.1093/jnci/80.13.1027
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Fidler,IJ
中科院分区：
文献类型：
--
作者：
Schackert,G;Simmons,RD;Buzbee,TM;Hume,DA;Fidler,IJ
本研究的目的是研究实验性脑转移瘤中血脑屏障的性质。将同基因纤维肉瘤或黑色素瘤细胞注射到小鼠的颈内动脉。几周后，一旦建立了实验性脑转移瘤，小鼠静脉注射荧光素钠，转移灶内的毛细血管增大且不规则，但荧光素钠未见渗漏，表明血脑屏障完整。巨噬细胞特异性抗原F4/80、Ⅱ类主要组织相容性复合体(MHC)抗原和巨噬细胞产物白介素1(IL-1)免疫组织化学定位显示肿瘤组织内有单核巨噬细胞浸润。转移灶含有大量表达F4/80和MHC II类抗原的星状巨噬细胞，但很少表达IL-1。肿瘤内可见圆形单核细胞样F4/80和MHC II类阳性细胞，并附着于肿瘤微血管壁上。纤维肉瘤脑转移的小鼠在远离转移灶的部位也有水肿性病变，其中含有大量表达II类MHC但不表达F4/80抗原的星形胶质细胞。总而言之，实验性脑转移瘤的血脑屏障是完整的，但单核细胞来源的巨噬细胞可以渗入病变。《癌症杂志》1988；80：1027-1034
The purpose of this study was to examine the nature of the bllod-brain barrier in experimental brain metastases. Syngeneic fibrosarcoma or melanoma cells were injected into the internal carotid arteries of mice. Several weeks later, once the experimental brain metastases were established, the mice were given injections iv of sodium fluorescein.The capillaries within the metastatic foci were enlarged and irregular, but there was no leakage of sodium fluorescein, showing that the blood-brain barrier was intact. The neoplastic lesions were infiltrated by mononuclear phagocytes, which were identified by immunohistochemical localization of the macrophage-specific antigen F4/80, class II major histocom-patibility complex (MHC) antigens, and the macrophage product interleukin-1(IL-1). The metastatic foci contained numerous stellate macrophages that expressed F4/80 and MHC class II antigens, but little IL-1. Round, Monocyte-like F4/80 and MHC class II-positive cells were also observed within the tumor lesions and adhering to walls of the tumor microvasculature. Mice with fibrosarcoma brain metastases also had edematous lesions at sites remote from the metastatic foci that contained numerous astrocytes expressing class II MHC but not F4/80 antigens. In conclusion, the blood-brain barrier is intact within experimental brain metastases, yet macrophages of blood monocyte origin can infiltrage the lesions. [J Natl Cancer Inst 1988;80:1027–1034]