Identification and characterization of two novel human papillomavi ruses (HPVs) by overlapping PICR: HPV102 and HPV106
Identification and characterization of two novel human papillomavi ruses (HPVs) by overlapping PICR: HPV102 and HPV106
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DOI：
10.1099/vir.0.83178-0
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Burk, Robert D.
中科院分区：
文献类型：
--
作者：
Chen, Zigui;Schiffman, Mark;Burk, Robert D.
从1例巴氏试验阳性的49岁西裔妇女和1例巴氏试验诊断为不典型鳞状细胞(ASCUS)的42岁西裔妇女的宫颈阴道细胞中分别扩增和克隆了HPV102(8078bp)和HPV106(8035bp)全基因组。HPV102和HPV106与HPV83(84.1%的同源性)和HPV90(83.5%的同源性)的同源性最高，分别属于生殖器HPV组、乳头瘤病毒种α3和α15。HPV102和HPV106包含5个早期基因(E6、E7、E1、E2和E4)和2个晚期基因(L2和L1)，均缺乏E5 ORF。根据系统发育分析和现有的临床信息，这两种新的HPV类型扩大了在下生殖道检测到的HPV的异质性。
Complete genomes of HPV102 (8078 bp) and HPV106 (8035 bp) were PCR amplified and cloned from cervicovaginal cells of a 49-year-old Hispanic female with reactive changes on her Pap test and a 42-year-old Hispanic female with a Pap test diagnosis of atypical squamous cells of unknown significance (ASCUS), respectively. The nucleotide sequence similarity of the complete L1 open reading frame (ORF) determined that HPV102 and HPV1 06 are most closely related to HPV83 (84.1 % identity) and HPV90 (83.5 % identity), respectively, placing them in the genital HPV groups, papillomaviruses species alpha 3 and alpha 15. HPV1 02 and HPV1 06 contain five early genes (E6, E7, E1, E2, and E4) and two late genes (L2 and L1), and both lack an E5 ORF. On the basis of phylogenetic analyses and available clinical information, these two novel HPV types expand the heterogeneity of HPVs detected in the lower genital tract.