Study of Demineralized Dental Enamel Treated with Different Fluorinated Compounds by Raman Spectroscopy.
Study of Demineralized Dental Enamel Treated with Different Fluorinated Compounds by Raman Spectroscopy.
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DOI：
10.31661/jbpe.v0i0.2003-1089
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发表时间：
2020-10
影响因子：
--
通讯作者：
M A AT
中科院分区：
文献类型：
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作者：
C C BO;A R VO;R Y SB;M A AT
目前用于釉质龋检测的诊断方法不能在非常早期的阶段检测龋损伤（初期龋损伤）。 本研究旨在利用拉曼光谱表征来确定三种含氟化合物对脱矿牙釉质的矿化效果。 在这项实验研究中，60个阻生第三磨牙，完整的解剖冠最近提取，没有结构缺陷，纵向切片的近远中方向使用金刚石光盘，获得两个工作面（颊和舌）。将获得的120个工作表面在37°C下浸入脱矿质溶液中96小时，以使釉质表面脱矿质。将所有样品随机分为三组（n=30），分别用银二胺氟化物（SDF）、二氟硅烷（DSF）和酸性磷酸氟化物（APF）处理样品表面，对照组（CG）不进行处理。将样品分别浸入pH 4.4和pH 7.0的交替溶液中进行脱矿和再矿化。采用主成分分析（PCA）对结果进行分析，以确定方差。 PCA 1与PCA 2在APF组中的差异最大（91.7%），其次是DSF（91.5%）和SDF（76.3%）。因此，三个实验组可以观察到牙釉质中更大的矿化。 除SDF外，APF和DSF均具有恢复牙釉质矿化的作用。在所有的次表层中鉴定了功能基团OH-和PO 43-。
Current diagnostic methods for enamel caries detection are unable to detect caries lesions (incipient caries lesion) at a very early stage. This study aims to determine the remineralizing effect of three fluorinated compounds on demineralized subsurface tooth enamel using Raman spectroscopy characterization. In this experimental study, sixty impacted 3rd molars, with intact anatomical crowns recently extracted and without structural defects, were sectioned longitudinally in a mesio-distal direction using a diamond disc, obtaining two working surfaces (buccal and lingual). The 120 working surfaces obtained were immersed for 96 h in a demineralization solution at 37°C in order to demineralize the enamel surface. All samples were randomly divided into three groups (n=30 each) and their surfaces were treated with silver diamine fluoride (SDF), Difluoride silane (DSF), and acidulated phosphate fluoride (APF), and with no treatment undertaken in the control group (CG). The samples were immersed in alternating solutions for demineralization and remineralization at pH 4.4 and pH 7.0, respectively. The results were analyzed with Principal Component Analysis (PCA) in order to determine the variance. The most important difference (91.7%) is observed in APF group between PCA1 respect to PCA2, followed by DSF (91.5%) and SDF (76.3%) respectively. Therefore, a greater remineralization in the dental enamel can be observed by the three experimental groups. The APF and DSF have the effect of recovering the mineralization of dental enamel, except for the SDF. Functional groups OH- and PO43- were identified in all subsurface.