Coronary Atherosclerosis Is Associated With Macrophage Polarization in Epicardial Adipose Tissue
Coronary Atherosclerosis Is Associated With Macrophage Polarization in Epicardial Adipose Tissue
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DOI：
10.1016/j.jacc.2011.01.048
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发表时间：
2011-07-12
影响因子：
24
通讯作者：
Sata, Masataka
中科院分区：
文献类型：
--
作者：
Hirata, Yoichiro;Tabata, Minoru;Sata, Masataka
目的探讨冠心病患者心外膜脂肪组织中巨噬细胞极化与动脉粥样硬化的关系。但人心外膜脂肪组织中巨噬细胞表型的改变及其在动脉粥样硬化形成中的作用尚不清楚。心脏手术（CAD，n = 38;非CAD，n = 40）。免疫组化法检测M1/M2巨噬细胞的浸润情况。结果冠心病患者心外膜脂肪中巨噬细胞浸润及促炎细胞因子和抗炎细胞因子的表达均较非冠心病患者明显增强（p < 0.05）。M1/M2比值与冠状动脉病变程度呈正相关（r = 0.312，p = 0.039）。CAD患者心外膜脂肪组织中促炎细胞因子的表达与M1/M2巨噬细胞比例呈正相关，而抗炎细胞因子的表达与M1/M2巨噬细胞比例呈负相关。结论冠心病患者心外膜脂肪组织中M1/M2巨噬细胞的比例较非冠心病患者发生了变化。人冠状动脉粥样硬化与心外膜脂肪组织巨噬细胞极化相关。（J Am科尔心脏病学杂志2011;58：248-55）（C）美国心脏病学会基金会2011年
Objectives The purpose of this report was to assess the link between macrophage polarization in epicardial adipose tissue and atherosclerosis in patients with coronary artery disease (CAD).Background Macrophage accumulation enhances chronic inflammation in adipose tissue, but macrophage phenotypic change in human epicardial adipose tissue and its role in atherogenesis are unknown.Methods Samples were obtained from epicardial and subcutaneous adipose tissue during elective cardiac surgery (CAD, n = 38; non-CAD, n = 40). Infiltration of M1/M2 macrophages was investigated by immunohistochemical staining with antibodies against CD11c and CD206, respectively. Expression of pro-and anti-inflammatory adipocytokines in adipose tissue was evaluated by real-time quantitative polymerase chain reaction.Results Infiltration of macrophages and expression of pro-and anti-inflammatory cytokines were enhanced in epicardial fat of patients with CAD compared with that in non-CAD patients (p < 0.05). The ratio of M1/M2 macrophages was positively correlated with the severity of CAD (r = 0.312, p = 0.039). Furthermore, the expression of pro-inflammatory cytokines was positively correlated, and the expression of anti-inflammatory cytokines was negatively correlated with the ratio of M1/M2 macrophages in epicardial adipose tissue of CAD patients. By contrast, there was no significant difference in macrophage infiltration and cytokine expression in subcutaneous adipose tissue between the CAD and non-CAD groups.Conclusions The ratio of M1/M2 macrophages in epicardial adipose tissue of CAD patients is changed compared with that in non-CAD patients. Human coronary atherosclerosis is associated with macrophage polarization in epicardial adipose tissue. (J Am Coll Cardiol 2011;58:248-55) (C) 2011 by the American College of Cardiology Foundation