Glycoprotein micelles isolated from vesicular stomatitis virus spontaneously partition into sonicated phosphatidylcholine vesicles.

Glycoprotein micelles isolated from vesicular stomatitis virus spontaneously partition into sonicated phosphatidylcholine vesicles.
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从水泡性口炎病毒中分离的糖蛋白胶束自发地分成超声处理的磷脂酰胆碱囊泡。

DOI:
10.1016/0042-6822(80)90323-2
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发表时间:
1980
期刊:
影响因子:
3.7
通讯作者:
Wagner,RR
Wagner,RR
中科院分区:
医学3区
文献类型:
--
作者:
PetriJr,WA;Wagner,RR

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水泡性口炎病毒的糖蛋白经辛基葡萄糖苷提取分离,不含洗涤剂和磷脂,在37°、10mMTris(pH 7.5)中孵育时,由糖蛋白形成的胶束自发地分割成预先形成的卵磷脂酰胆碱囊泡。当糖蛋白插入囊泡双层时,糖蛋白的疏水尾部片段变得不能被酶消化。用[~3H]棕榈酸酯活体标记糖蛋白的共价结合脂肪酸时,证明该脂肪酸结合在囊泡和病毒粒子膜中的疏水性糖蛋白尾段上。
Micelles formed by glycoprotein of vesicular stomatitis virus, isolated by octylglucoside extraction and purified free of detergent and phospholipid, spontaneously partitioned into preformed sonicated egg phosphatidylcholine vesicles when the glycoprotein rosettes and vesicles were incubated together in 10 mMTris (pH 7.5) at 37°. The hydrophobic tail fragment of the glycoprotein became resistant to protease digestion when the glycoprotein was inserted into the vesicle bilayer. When the covalently attached fatty acid of the glycoprotein was labeledin vivowith [3H]lpalmitate, it was demonstrated that the fatty acid was attached to the hydrophobic glycoprotein tail fragment in vesicle and virion membranes.
DOI: 10.1128/jvi.7.1.59-70.1971
发表时间: 1971-01-01
影响因子: 5.4
作者:
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通讯作者: WAGNER, RR
DOI: 10.1016/0042-6822(79)90502-6
发表时间: 1979
期刊: Virology
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DOI: 10.1111/j.1432-1033.1974.tb03599.x
发表时间: 1974-01-01
期刊: EUROPEAN JOURNAL OF BIOCHEMISTRY
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作者:
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通讯作者: LASKEY, RA
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发表时间: 1978
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