A new rat model of prenatal bowel obstruction: development and early assessment
A new rat model of prenatal bowel obstruction: development and early assessment
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DOI：
10.1016/j.jpedsurg.2009.07.031
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发表时间：
2010-03-01
影响因子：
2.4
通讯作者：
Sturtz, Franck G.
中科院分区：
文献类型：
--
作者：
Fourcade, Laurent M.;Mousseau, Yoanne;Sturtz, Franck G.
目的：虽然肠动力障碍常常使先天性肠闭锁新生儿的术后外科治疗复杂化，但其发病机制仍不清楚。产前肠梗阻的动物模型主要建立在羔羊和鸡上。尽管这些模型带来了新的见解，但它们有一个或多个局限性，如高胎儿死亡率，高成本，妊娠期长，每窝胎儿数量不足。方法：建立大鼠产前肠梗阻模型，观察肠梗阻引起的组织学改变。我们的报告，技术设备和第一次评估，这种闭锁模型的说教的方式，让其他研究人员很容易地重现model.Results：产前肠梗阻在这项研究中满足所有的宏观和组织学标准，通常列出的其他模型的产前肠梗阻，已在其他物种。此外，我们的模型，我们获得了很高的成功率，在低cost.Conclusions：我们在这项研究中提出了一个可重复的模型，产前肠梗阻的大鼠与产前肠梗阻的宏观和组织学特征。(C)2010年爱思唯尔公司All rights reserved.
Purpose: Although intestinal motility disorders often complicate the postoperative surgical management of newborns with congenital intestinal atresia, their pathogenesis remains unclear. Animal models of prenatal intestinal obstruction have been mainly developed in the lamb and the chicken. Despite new insights brought by these models, they have one or more limitations, such as high fetal mortality rates, high costs, long gestation periods, and an insufficient number of fetuses per litter. Moreover, some species are phylogenetically distant from mammals.Methods: We developed a reproducible model of prenatal intestinal obstruction in the rat to study the histologic changes induced by the obstruction. We report, the technical devices and the first assessment of this atresia model in a didactic way to allow other researchers to easily reproduce the model.Results: Prenatal intestinal obstructions in this study fulfilled all the macroscopic and histologic criteria usually listed by other models of prenatal intestinal obstruction that have been developed in other species. Furthermore with our model, we obtained a high success rate at a low cost.Conclusions: We presented in this study a reproducible model of prenatal intestinal obstruction in the rat with the macroscopical and histologic features of prenatal intestinal obstruction. (C) 2010 Elsevier Inc. All rights reserved.