Insights into the transcriptomics of polyphagy: Bemisia tabaci adaptability to phenylpropanoids involves coordinated expression of defense and metabolic genes
Insights into the transcriptomics of polyphagy: Bemisia tabaci adaptability to phenylpropanoids involves coordinated expression of defense and metabolic genes
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DOI：
10.1016/j.ibmb.2011.12.007
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发表时间：
2012-04-01
影响因子：
3.8
通讯作者：
Morin, Shai
中科院分区：
文献类型：
--
作者：
Alon, Michal;Elbaz, Moshe;Morin, Shai
烟粉虱Bemisia tabaci是一种世界性的农业害虫。尽管它的重要性，防御机制的分子基础在B。烟粉虱对主要植物次生防御化合物如苯丙素类的抗性仍是未知的。我们的实验系统利用了组成型表达PAP 1/AtMYB 75转录因子的转基因烟草植物，该转录因子相对特异性地激活苯丙素类/类黄酮生物合成途径。本研究采用抑制性消减杂交（SSH）和cDNA微阵列技术比较了B细胞基因表达的差异。烟粉虱成虫与野生型或转基因植物接触6小时。共对来自SSH文库的2880个克隆进行测序。SSH和cDNA微阵列分析均表明B之间存在复杂的相互作用。烟粉虱和次生防御代谢产物产生的phenylproapnoids/黄酮途径，涉及增强表达的解毒，免疫，氧化应激和一般的压力相关基因以及一般代谢和核糖体基因。定量实时PCR显示显着变化，这些基因的表达中的几个响应喂养人工饲料中含有黄酮类化合物槲皮素。转录活性的提高并不伴随着繁殖性能的降低，表明B的高适应性。烟粉虱这一大群植物次生防御代谢产物。(C)2011爱思唯尔有限公司保留所有权利。
The whitefly Bemisia tabaci is a major generalist agricultural pest of field and horticultural crops worldwide. Despite its importance, the molecular bases of defense mechanisms in B. tabaci against major plant secondary defense compounds, such as the phenylpropanoids, remain unknown. Our experimental system utilized transgenic Nicotiana tabacum plants constitutively expressing the PAP1/AtMYB75 transcription factor which activates relatively specifically the phenylpropanoid/flavonoids biosynthetic pathway. Our study used suppression subtractive hybridization (SSH) and cDNA microarray approaches to compare gene expression between B. tabaci adults subjected to wild-type or transgenic plants for 6 h. A total of 2880 clones from the SSH libraries were sequenced. Both the SSH and cDNA microarray analyses indicated a complex interaction between B. tabaci and secondary defense metabolites produced by the phenylproapnoids/flavonoids pathway, involving enhanced expression of detoxification, immunity, oxidative stress and general stress related genes as well as general metabolism and ribosomal genes. Quantitative real-time PCR revealed significant changes in the expression of several of these genes in response to feeding on artificial diet containing the flavonoids quercetin. The elevated transcriptional activity was not accompanied by reduced reproductive performance, indicating high adaptability of B. tabaci to this large group of plant secondary defense metabolites. (C) 2011 Elsevier Ltd. All rights reserved.