Time-Lapse Mechanical Characterization of Zona Pellucida Using a Cell Carrier Chip
Time-Lapse Mechanical Characterization of Zona Pellucida Using a Cell Carrier Chip
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DOI：
10.1109/jmems.2018.2818183
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发表时间：
2018-04
影响因子：
2.7
通讯作者：
K. Nakahara;S. Sakuma;M. Kawahara;Masashi Takahashi;F. Arai
中科院分区：
文献类型：
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作者：
K. Nakahara;S. Sakuma;M. Kawahara;Masashi Takahashi;F. Arai
本文的目的是测量卵母细胞透明带(ZP)的力学特性随时间的变化。为了测量培养过程中的杨氏模数等力学特性，需要实现对目标卵母细胞的培养和力学特性的测量。通常，测量系统是建立在显微镜上来观察卵母细胞的变形，但这种系统很难与传统的孵化器集成在一起。因此，我们提出了一种用于时移力学表征的细胞载体芯片。细胞载体芯片有一个探头和一个力传感器来测量ZP的机械特性。此外，芯片使我们能够将目标卵母细胞从测量系统携带到孵化器。采用弹性球壳模型，分别在解冻后0.5、1、2、3、4、5、6h测量冷冻后卵母细胞ZP的杨氏模数，计算ZP的杨氏模数。结果表明，随着培养时间的延长，ZP的杨氏模数有增大的趋势。[2017-0133]
The aim of this paper is to measure the changes in the mechanical characteristics of the zona pellucida (ZP) of an oocyte over time. To measure the mechanical characteristics such as Young’s modulus during cultivation, it is necessary to achieve the cultivation and the measurement of the mechanical characteristics of target oocytes. Generally, measurement systems are constructed on a microscope for observing the deformation of the oocytes, but it is difficult to integrate such a system with a conventional incubator. Therefore, we propose a cell carrier chip for the time-lapse mechanical characterization. The cell carrier chip has a probe as well as a force sensor to measure the mechanical characteristics of the ZP. In addition, the chip enables us to carry the target oocyte from the measurement system to the incubator. To calculate the Young’s modulus of the ZP, we used an elastic spherical shell model and measured the Young’s modulus of the ZP of cryopreserved oocytes at 0.5, 1, 2, 3, 4, 5, and 6 h after being thawed. The result indicates that the Young’s modulus of the ZP tends to increase with the cultivation time. [2017–0133]