Evaluation of glioma-associated oncogene 1 expression and its correlation with the expression of sonic hedgehog, E-cadherin and S100a4 in human hepatocellular carcinoma
Evaluation of glioma-associated oncogene 1 expression and its correlation with the expression of sonic hedgehog, E-cadherin and S100a4 in human hepatocellular carcinoma
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人肝细胞癌中胶质瘤相关癌基因1的表达及其与声波刺猬蛋白、E-钙粘蛋白和S100a4表达的相关性评估
DOI：
10.3892/mmr.2010.375
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发表时间：
2010-11-01
影响因子：
3.4
通讯作者：
Liu, Qingguang
中科院分区：
文献类型：
--
作者：
Zheng, Xin;Yao, Yinmin;Liu, Qingguang
本研究的目的是分析胶质瘤相关癌基因1 （Glil）在肝细胞癌（HCC）中的mRNA和蛋白表达及其与hedgehog基因（Hh）信号配体之一的超音hedgehog基因（Shh）和两种上皮-间质转化（EMT）标志物E-cadherin和S100a4的相关性。我们还研究了HCC中Hh信号和EMT之间的潜在串扰。从30例原发性HCC患者新鲜采集成对的HCC和正常肿瘤邻近组织。通过逆转录聚合酶链反应和免疫组织化学检测Glil mRNA和蛋白水平的表达。免疫组织化学检测Shh、E-cadherin和S100a4蛋白表达与Glil的相关性。与正常癌旁组织相比，HCC肿瘤组织中Gill mRNA和蛋白的表达明显上调(P
The aim of the present study was to analyze the mRNA and protein expression of glioma-associated oncogene 1 (Glil) in hepatocellular carcinoma (HCC) and its correlation with sonic hedgehog (Shh), one of the ligands of hedgehog (Hh) signaling, and two epithelial-mesenchymal transition (EMT) markers, E-cadherin and S100a4. We also investigated the potential crosstalk between Hh signaling and EMT in HCC. Paired HCC and normal tumor-adjacent tissues were freshly collected from 30 primary HCC patients. Glil expression at both the mRNA and protein level was examined by reverse transcription-polymerase chain reaction and immunohistochemistry. The protein expression of Shh, E-cadherin and S100a4 was evaluated by immunohistochemistry to identify correlations with Glil. The mRNA and protein expression of Gill was significantly up-regulated in the HCC tumor tissues compared to the normal tumor-adjacent tissues (P