The dim light melatonin onset, melatonin assays and biological rhythm research in humans.
The dim light melatonin onset, melatonin assays and biological rhythm research in humans.
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人类的弱光褪黑激素起效、褪黑激素测定和生物节律研究。
DOI：
10.1159/000014573
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Lewy,AJ
中科院分区：
文献类型：
--
作者：
Lewy,AJ
对于人类昼夜节律相位位置最有用的标记是昏暗光褪黑激素起始（DLMO）。最好在晚上每隔30分钟或更短时间采集一次血液或唾液样本。环境光强度不应超过30-50 lx。多年来，DLMO的测定主要是用“金标准”GCMS技术来测量人血浆中的褪黑激素。然而，新的和改进的RIA现在提供了必要的灵敏度和准确性（特异性），用于检测白天低水平开始在晚上增加的时间：DLMO的操作阈值越低，它作为相位标记物越可靠。
The most useful marker for human circadian phase position is the dim light melatonin onset (DLMO). This is optimally obtained by sampling blood or saliva in the evening at intervals of 30 min or less. Ambient light intensity should not exceed 30–50 lx. For many years, the DLMO was determined mainly with the ‘gold standard’ GCMS technique for measuring melatonin in human plasma. However, new and improved RIAs now provide the requisite sensitivity and accuracy (specificity) for detecting the time that low daytime levels begin to increase in the evening: the lower the operational threshold for the DLMO, the more reliable it is as a phase marker.