Modular multi-level circuits from immobilized DNA-Based logic gates
Modular multi-level circuits from immobilized DNA-Based logic gates
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DOI：
10.1021/ja0710149
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发表时间：
2007-12-05
影响因子：
15
通讯作者：
Ghadiri, M. Reza
中科院分区：
文献类型：
--
作者：
Frezza, Brian M.;Cockroft, Scott L.;Ghadiri, M. Reza
分子计算的基本目标之一是再现数字逻辑的原则，例如组件模块化和分层电路设计。实现这一目标的重要一步是创造分子逻辑门，可以合理地连接到多级电路。在这里，我们报告。设计和功能特性的一套完整的模块化DNA为基础的布尔逻辑门（与，或，和与非），并进一步展示了他们的布线到一个三电平电路，展示布尔异或（异或）功能。该方法基于固体支持的DNA逻辑门，其设计用于与单链DNA输入和输出一起操作。由于溶液相作为门之间的通信介质，因此可以通过将一个门的DNA输出指定为另一个门的输入来实现电路布线。固体支持的逻辑门通过显著简化选择用于构建多级电路的适当DNA输入和输出序列的任务，提供了与解相系统相比增强的门模块化。本文报道的分子逻辑门和电路的特征在于将DNA输出耦合到单输入REPORT门并监测所产生的荧光输出信号。
One of the fundamental goals of molecular computing is to reproduce the tenets of digital logic, such as component modularity and hierarchical circuit design. An important step toward this goal is the creation of molecular logic gates that can be rationally wired into multi-level circuits. Here we report the. design and functional characterization of a complete set of modular DNA-based Boolean logic gates (AND, OR, and AND-NOT) and further demonstrate their wiring into a three-level circuit that exhibits Boolean XOR (exclusive OR) function. The approach is based on solid-supported DNA logic gates that are designed to operate with single-stranded DNA inputs and outputs. Since the solution-phase serves as the communication medium between gates, circuit wiring can be achieved by designating the DNA output of one gate as the input to another. Solid-supported logic gates provide enhanced gate modularity versus solution-phase systems by significantly simplifying the task of choosing appropriate DNA input and output sequences used in the construction of multi-level circuits. The molecular logic gates and circuits reported here were characterized by coupling DNA outputs to a single-input REPORT gate and monitoring the resulting fluorescent output signals.