INTERACTIONS BETWEEN DNA-BOUND TRIMERS OF THE YEAST HEAT-SHOCK FACTOR
INTERACTIONS BETWEEN DNA-BOUND TRIMERS OF THE YEAST HEAT-SHOCK FACTOR
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DOI：
10.1128/mcb.14.1.501
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发表时间：
1994-01-01
影响因子：
5.3
通讯作者：
FACKENTHAL, DL
中科院分区：
文献类型：
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作者：
BONNER, JJ;BALLOU, C;FACKENTHAL, DL
热休克转录因子（HSF）是一种三聚体，与含有nGAAn序列反向重复序列的DNA结合。HSF可以与序列nGAAnnTTCn或序列nTTCnnGAAn结合DNA，对任一序列的偏好性都很小。然而，（nGAAnnTTCn）2作为热冲击响应元件（HSE）的活性明显低于（nTTCnnGAAn）2。DNA-蛋白质复合物的电泳迁移率和蛋白质单体之间的化学交联表明序列（nGAAnnTTCn）2能够结合单个HSF三聚体。相比之下，具有更高生物活性的序列（nTTCnnGAAn）2能够结合两个三聚体。因此，四个nGAAn元素HSE结合一个或两个三聚体的能力取决于元素所呈现的排列。对自然发生的HSE的调查显示序列（nTTCnnGAAn）2是更普遍的。我们建议，一个排列的能力更大的其他绑定两个HSF三聚体帐户的初始识别自然发生的热休克共识序列作为一个区域的二分体对称。
The heat shock transcription factor (HSF) is a trimer that binds to DNA containing inverted repeats of the sequence nGAAn. HSF can bind DNA with the sequence nGAAnnTTCn or with the sequence nTTCnnGAAn, with little preference for either sequence over the other. However, (nGAAnnTTCn)2 is considerably less active as a heat shock response element (HSE) than is (nTTCnnGAAn)2. The electrophoretic mobilities of DNA-protein complexes and chemical cross-linking between protein monomers indicate that the sequence (nGAAnnTTCn)2 is capable of binding a single HSF trimer. In contrast, the sequence with higher biological activity, (nTTCnnGAAn)2, is capable of binding two trimers. Thus, the ability of four-nGAAn-element HSEs to bind one or two trimers depends on the permutation with which the elements are presented. A survey of naturally occurring HSEs shows the sequence (nTTCnnGAAn)2 to be the more prevalent. We suggest that the greater ability of one permutation over the other to bind two HSF trimers accounts for the initial identification of the naturally occurring heat shock consensus sequence as a region of dyad symmetry.