A novel glucose biosensor based on the immobilization of glucose oxidase onto gold nanoparticles-modified Pb nanowires
A novel glucose biosensor based on the immobilization of glucose oxidase onto gold nanoparticles-modified Pb nanowires
复制标题
一种基于金纳米粒子修饰的铅纳米线上固定葡萄糖氧化酶的新型葡萄糖生物传感器
DOI：
10.1016/j.bios.2009.06.022
复制
发表时间：
2009-09-15
影响因子：
12.6
通讯作者：
Chen, Qiang
中科院分区：
文献类型：
--
作者：
Wang, Huicai;Wang, Xinsheng;Chen, Qiang
基于葡萄糖氧化酶（GOD）在牛血清白蛋白（BSA）基质中的交联固定，制备了一种新型的葡萄糖生物传感器，并将其修饰在铂电极上，以金纳米颗粒修饰铅纳米线（GNPs-Pb NWs）。采用L-半胱氨酸辅助自组装法制备了铅纳米线，并通过-SH-Au特异性相互作用将金纳米粒子（GNP）固定在铅纳米线表面。通过透射电子显微镜（TEM）检查GNPs-Pb纳米线的形态特征。循环伏安法和计时电流法用于研究和优化所得生物传感器的电化学性能。Pb纳米线和GNP的协同效应使该传感器表现出优异的电催化活性和对葡萄糖的良好响应性能。系统地研究了pH和外加电位对该传感器安培响应的影响。在pH7.0时，该传感器的灵敏度为135.5 μ A mW（-1）cm（-2），检测限为2 μ M（S/N = 3），响应时间为20 min
A novel glucose biosensor was developed, based on the immobilization of glucose oxidase (GOD) with cross-linking in the matrix of bovine serum albumin (BSA) on a Pt electrode, which was modified with gold nanoparticles decorated Pb nanowires (GNPs-Pb NWs). Pb nanowires (Pb NWs) were synthesized by an L-Cysteine-assisted self-assembly route, and then gold nanoparticles (GNPs) were attached onto the nanowire surface through -SH-Au specific interaction. The morphological characterization of GNPs-Pb NWs was examined by transmission electron microscopy (TEM). Cyclic voltammetry and chronoamperometry were used to study and to optimize the electrochemical performance of the resulting biosensor. The synergistic effect of Pb NWs and GNPs made the biosensor exhibit excellent electrocatalytic activity and good response performance to glucose. The effects of pH and applied potential on the amperometric response of the biosensor have been systemically studied. In pH 7.0, the biosensor showed the sensitivity of 135.5 mu A mW(-1) cm(-2), the detection limit of 2 mu M (S/N = 3), and the response time