A POLY(METHYL METHACRYLATE METHOD FOR LARGE SPECIMENS OF MINERALIZED BONE WITH IMPLANTS
A POLY(METHYL METHACRYLATE METHOD FOR LARGE SPECIMENS OF MINERALIZED BONE WITH IMPLANTS
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DOI：
10.3109/10520298709108030
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发表时间：
1987-11-01
期刊：
影响因子：
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通讯作者：
BLOEBAUM, RD
中科院分区：
文献类型：
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作者：
EMMANUAL, J;HORNBECK, C;BLOEBAUM, RD
描述了一种简单的改良聚甲基丙烯酸甲酯方法，用于具有植入物和生物活性材料的大型矿化骨样本，该方法对骨和植入物之间的界面产生一致的良好组织学保存。将含有由羟基磷灰石、光滑聚乙烯、多孔聚乙烯和碳组成的植入物的人股骨头、整个兔髁和犬胫骨和股骨在递增等级的乙醇中脱水，并在自动组织处理机上用二甲苯清洗，该组织处理机交替真空和压力22小时。用洗涤过的聚甲基丙烯酸甲酯在4℃真空下进行渗透13天。聚合在38℃的广口玻璃罐中进行36小时，使得总处理时间少于20天。唯一重要的改性是聚甲基丙烯酸甲酯，它比平常使用更少的增塑剂，以获得更硬的嵌段。这使得能够生产 4 μm 切片，并良好地保存矿化和细胞区域，用于代谢性骨疾病、形态测量、荧光染料染色和植入物原位界面分析的研究。
A simple modified polymethyl methacrylate method is described for large mineralized bone specimens with implants and bioactive materials which produces consistently good histological preservation of the interface between bone and implant. Human femoral heads, whole rabbit condyles and canine tibias and femurs containing implants consisting of hydroxyapatite, smooth polyethylene, porous polyethylene and carbon were dehydrated in ascending grades of ethanol and cleared with xylene on an automated tissue processor which alternated vacuum and pressure for 22 hr. Infiltration was done with washed polymethyl methacrylate at 4 C under vacuum for 13 days. Polymerization was carried out in wide-mouth glass jars ar 38 C for 36 hr so that the total processing time was less than 20 days. The only important modification was in the polymethyl methacrylate, which ahd less plasticizer than usual in order to give a harder block. This enabled production of 4 .mu.m sections with good preservation of mineralized and cellular areas for the study of metabolic bone diseases, morphometry, fluorochrome lavelling and interface analysis with the implant in situ.