Isoenzyme pattern of superoxide dismutase in different varieties of citrus plants
Isoenzyme pattern of superoxide dismutase in different varieties of citrus plants
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DOI：
10.1111/j.1399-3054.1989.tb05479.x
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发表时间：
1989-08
影响因子：
6.4
通讯作者：
M. Almansa;L. A. Río;C. Alcaraz;F. Sevilla
中科院分区：
文献类型：
--
作者：
M. Almansa;L. A. Río;C. Alcaraz;F. Sevilla
使用聚丙烯酰胺凝胶中的等电聚焦来表征不同柑橘植物品种叶子中的超氧化物歧化酶（SOD；EC 1.15.1.1）。研究的植物包括 Citrus limonum R.（变种 Verna、Fino 和 Eureka）、C. paradisi Mac（变种 Red Blush 和 Marsh）、C. aurantium L.（变种 Comun）、C. sinensis L. Osbeck（变种 Navel、Valencia 和 Salustiano）和 C. reticulata B.（变种 Satsuma）。 SOD 的三种分子形式因其对氰化物和 H2O2 的敏感性不同而彼此不同。柠檬草叶子中存在四种 Cu,Zn-SOD、三种 Fe-SOD 和两种 Mn-SOD。然而，在不同品种的中华枳实、天堂枳实、枳壳枳实和网枳实中，Fe-SOD同工酶的活性和数量均低于柠檬叶，而MN-SOD同工酶的数量则有所增加。 Cu,Zn-SODs 在不同物种和品种中没有表现出显着差异。在芸香科植物的几个物种中对 Fe-SOD 的鉴定扩展了少数高等植物，其中已证明这些含铁金属酶的存在。从进化的角度来看，获得的结果可能有用，也可用于使用 SOD 同工酶作为功能金属标记的矿物质营养研究。
Superoxide dismutases (SOD; EC 1.15.1.1) in leaves from different cultivars of citrus plants were characterized using isoelectric focusing in polyacrylamide gels. The plants studied included Citrus limonum R. (cvs Verna, Fino, and Eureka), C. paradisi Mac (cvs Red Blush and Marsh), C. aurantium L. (cv. Comun), C. sinensis L. Osbeck (cvs Navel, Valencia, and Salustiano), and C. reticulata B. (cv. Satsuma). The three molecular forms of SOD were distinguished from each other by their different sensitivity to cyanide and H2O2. In C. limonum leaves, four Cu,Zn-SODs, three Fe-SODs and two Mn-SODs were present. However, in leaves from different varieties of C. sinensis, C. paradisi, C. aurantium and C. reticulata the activity and number of Fe-SOD isoenzymes were lower than in lemon leaves, whereas the number of MN-SOD isozymes was increased. Cu,Zn-SODs did not show significant variations in the different species and cultivars. The identification of Fe-SODs in several species of the plant family Rutaceae extends the small number of higher plants where the presence of these Fe-containing metalloenzymes has been demonstrated. Results obtained may be useful from an evolutionary viewpoint and also in mineral nutrition studies using SOD isozymes as markers of functional metals.