A Pilot Study of Chicago Waterways as Reservoirs of Multidrug-Resistant Enterobacteriaceae (MDR-Ent) in a High-Risk Region for Community-Acquired MDR-Ent Infection in Children.
A Pilot Study of Chicago Waterways as Reservoirs of Multidrug-Resistant Enterobacteriaceae (MDR-Ent) in a High-Risk Region for Community-Acquired MDR-Ent Infection in Children.
复制标题
芝加哥水道作为儿童社区获得性 MDR-Ent 感染高风险地区耐多药肠杆菌科 (MDR-Ent) 储存库的初步研究。
DOI：
10.1128/aac.02310-19
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发表时间：
2020
影响因子：
4.9
通讯作者：
Pruden,Amy
中科院分区：
文献类型：
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作者：
Logan,LataniaK;Zhang,Liqing;Green,StefanJ;Dorevitch,Samuel;Arango-Argoty,GustavoA;Reme,Kendrick;Garner,Emily;Aldstadt,Jared;Johnson-Walker,YvetteJ;Hayden,MaryK;Weinstein,RobertA;Pruden,Amy
社区获得性耐多药细菌科（MDR-Ent）感染在美国持续增加。在之前的研究中，我们确定了伊利诺伊州芝加哥的邻近地区，那里的儿童感染耐多药耳鼻炎的几率是其他地区的5到6倍。为防止耐多药耳鼻喉病的社区传播，我们需要确定耐多药耳鼻喉病的水库。对芝加哥4条水道进行了耐多药ent及其相关抗生素耐药基因（ARGs）的初步研究。有三条水道（A1到A3）被标记为安全的“偶然接触娱乐”（例如，皮划艇），A4是非娱乐水道，携带未经消毒的水。收集地表水样品，进行标准细菌培养和散弹枪宏基因组测序。一般来说，A3和A4（相邻的水路没有水力连接）在肠杆菌科细菌分类群、ARG谱和相应分支和属的丰度上惊人地相似。此外，从整个微生物群落中恢复的总ARG丰度在A3和A4之间呈强相关（R2= 0.97）。A4（783个最可能数[MPN]）和A3（200个MPN）相对于A2（84个MPN）和A1（32个MPN）的大肠杆菌数量（每100毫升水）最高。我们在肠杆菌科发现了一些ARGs，如MCR-1（粘菌素）、Qnr和OqxA/B（喹诺酮类药物）、CTX-M、OXA和ACT/MIR （β -内酰胺类药物）和AAC（氨基糖苷类药物）。我们发现附近没有水力连接的水道之间的微生物群落组成存在显著相关性，这表明交叉播种和ARGs的迁移潜力。肠杆菌科和ARG谱支持了娱乐水道是社区获得性耐多药耳炎潜在来源的假设。
Community-acquired multidrug resistantEnterobacteriaceae(MDR-Ent) infections continue to increase in the United States. In prior studies, we identified neighboring regions in Chicago, Illinois, where children have 5 to 6 times greater odds of MDR-Ent infections. To prevent community spread of MDR-Ent, we need to identify the MDR-Ent reservoirs. A pilot study of 4 Chicago waterways for MDR-Ent and associated antibiotic resistance genes (ARGs) was conducted. Three waterways (A1 to A3) are labeled safe for “incidental contact recreation” (e.g., kayaking), and A4 is a nonrecreational waterway that carries nondisinfected water. Surface water samples were collected and processed for standard bacterial culture and shotgun metagenomic sequencing. Generally, A3 and A4 (neighboring waterways which are not hydraulically connected) were strikingly similar in bacterial taxa, ARG profiles, and abundances of corresponding clades and genera within theEnterobacteriaceae. Additionally, total ARG abundances recovered from the full microbial community were strongly correlated between A3 and A4 (R2= 0.97). Escherichia coli numbers (per 100 ml water) were highest in A4 (783 most probable number [MPN]) and A3 (200 MPN) relative to A2 (84 MPN) and A1 (32 MPN). We found concerning ARGs inEnterobacteriaceaesuch as MCR-1 (colistin), Qnr and OqxA/B (quinolones), CTX-M, OXA and ACT/MIR (beta-lactams), and AAC (aminoglycosides). We found significant correlations in microbial community composition between nearby waterways that are not hydraulically connected, suggesting cross-seeding and the potential for mobility of ARGs.Enterobacteriaceaeand ARG profiles support the hypothesized concerns that recreational waterways are a potential source of community-acquired MDR-Ent.