HOT KNIFE MICROTOMY FOR LARGE AREA SECTIONING AND COMBINED LIGHT AND ELECTRON-MICROSCOPY IN NEUROANATOMY AND NEUROPATHOLOGY
HOT KNIFE MICROTOMY FOR LARGE AREA SECTIONING AND COMBINED LIGHT AND ELECTRON-MICROSCOPY IN NEUROANATOMY AND NEUROPATHOLOGY
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DOI：
10.1007/bf01188034
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发表时间：
1990-10-01
期刊：
影响因子：
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通讯作者：
GOSZTONYI, G
中科院分区：
文献类型：
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作者：
MCGEERUSSELL, SM;DEBRUIJN, WC;GOSZTONYI, G
本文给出的技术细节满足了神经解剖学和神经病理学对方法的需求，这些方法将广泛的光学显微镜调查与灌注良好的脑材料中逻辑选择区域的详细超微结构研究相结合，并直接来自1956年美国国立卫生研究院Palay实验室的经验。使用推荐的方法将得到非常大面积的良好切片（高达和大于1 cm × 1cm）。cm）的完全硬化块，可在所有点进行电子显微镜检查。在这样大的块完全相关，结合光学和电子显微镜可以很容易地进行。这个过程被称为“热刀显微镜”。在三个不同的实验室中，通过灌注和热刀切片法进行的初级醛固定，从正常、患病和病毒感染的脑组织中获得了一致的优秀数据。这些技术允许全面的神经解剖控制和方向，使全面的相关映射整个中枢神经系统可行，并允许研究感染过程的时间过程。
The technical details given in this paper meet the demand in neuroanatomy and neuropathology for methods which combine broad light microscopical surveys with detailed ultrastructural studies of logically selected areas in well perfused brain material, and emerge directly from experiences in Palay''s laboratory at the National Institutes of Health in 1956. Using the procedures recommended will give good sections of exceptionally large areas (up to, and above 1 cm .times. cm) of fully hardened blocks available at all points for electron microscopy. On such large blocks fully correlative, combined light and electron microscopy may be carried out easily. The process is termed ''hot knife microtomy''. In three different laboratories, primary aldehyde fixation by perfusion and hot knife microtomy have given uniformly excellent data from normal, diseased, and virus-infected brain tissues. These techniques permit full neuroanatomical control and orientation, make comprehensive correlative mapping throughout the CNS feasible, and allow study of the time course of infective processes.