EMS Mutagenesis of Maize Pollen.
EMS Mutagenesis of Maize Pollen.
复制标题
玉米花粉的 EMS 诱变。
作者：
A. Settles
有效的诱变对于将感兴趣的性状与特定植物基因连接起来至关重要。玉米定点诱变和序列索引遗传资源的开发允许对单个基因进行有针对性的分析。这些反向遗传学方法仅研究与感兴趣的性状相关的候选基因，因此可能存在确认偏差。诱导的随机突变的遗传筛选对于识别新基因座以及已知突变基因座的相互作用因素非常重要。化学诱变提供非常高的突变率，可用于多种筛选设计。本章提供了使用石蜡或矿物油对玉米花粉进行甲磺酸乙酯 (EMS) 诱变的更新方案。诱变发生在成熟花粉中，导致胚乳和胚胎基因型不一致以及扇形 M1 植物。讨论了遗传筛选中这些因素的考虑因素。
Effective mutagenesis is critical for connecting traits of interest to specific plant genes. The development of site-directed mutagenesis and sequenced-indexed genetics resources in maize allows for targeted analysis of individual genes. These reverse genetics approaches have the potential for confirmation bias by only studying candidate genes for association with traits of interest. Genetic screens of induced, random mutations are important for identifying novel loci as well as interacting factors for known mutant loci. Chemical mutagenesis provides very high mutation rates and can be used for a variety of screen designs. This chapter provides an updated protocol for ethyl methanesulfonate (EMS) mutagenesis of maize pollen using paraffin or mineral oil. Mutagenesis occurs in mature pollen causing nonconcordant endosperm and embryo genotypes as well as sectored M1 plants. Considerations for these factors in genetic screens are discussed.