Reduction of GIGANTEA expression in transgenic Brassica rapa enhances salt tolerance
Reduction of GIGANTEA expression in transgenic Brassica rapa enhances salt tolerance
复制标题
DOI：
10.1007/s00299-016-2008-9
复制
发表时间：
2016-09-01
影响因子：
6.2
通讯作者：
Kim, WeoYeon
中科院分区：
文献类型：
--
作者：
Kim, Jin A.;Jung, Ha-eun;Kim, WeoYeon
本研究通过RNAi技术降低GIGANTEA（GI）基因的表达，增强了白菜对盐胁迫的耐受性。生物钟整合了环境信号和内部信号，以协调不同的生理输出。GIGANTEA（GI）基因由于其对光周期开花的重要贡献而首次被发现，并且已经被证明是植物昼夜节律钟的关键组成部分，并且有助于多种环境胁迫反应。我们发现，在芜菁（BrGI）的GI基因是相似的拟南芥GI的表达模式和功能。BrGI在功能上拯救了拟南芥gi-201功能缺失突变体的晚花表型。RNAi介导的拟南芥Col-0和大白菜中GI表达的抑制，B。萝卜DH 03耐盐性增强。我们的研究结果表明，拟南芥中描述的GI的分子功能在B中是保守的。并表明通过RNAi和转基因过表达操纵基因表达可以增强对非生物胁迫的耐受性，从而提高农作物产量。
Here we report the enhancement of tolerance to salt stress in Brassica rapa (Chinese cabbage) through the RNAi-mediated reduction of GIGANTEA ( GI ) expression.Circadian clocks integrate environmental signals with internal cues to coordinate diverse physiological outputs. The GIGANTEA (GI) gene was first discovered due to its important contribution to photoperiodic flowering and has since been shown to be a critical component of the plant circadian clock and to contribute to multiple environmental stress responses. We show that the GI gene in Brassica rapa (BrGI) is similar to Arabidopsis GI in terms of both expression pattern and function. BrGI functionally rescued the late-flowering phenotype of the Arabidopsis gi-201 loss-of-function mutant. RNAi-mediated suppression of GI expression in Arabidopsis Col-0 and in the Chinese cabbage, B. rapa DH03, increased tolerance to salt stress. Our results demonstrate that the molecular functions of GI described in Arabidopsis are conserved in B. rapa and suggest that manipulation of gene expression through RNAi and transgenic overexpression could enhance tolerance to abiotic stresses and thus improve agricultural crop production.