ZmMPK17, a novel maize group D MAP kinase gene, is involved in multiple stress responses
ZmMPK17, a novel maize group D MAP kinase gene, is involved in multiple stress responses
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ZmMPK17 是一种新型玉米 D 组 MAP 激酶基因，参与多种应激反应
DOI：
10.1007/s00425-011-1510-0
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发表时间：
2012-04-01
期刊：
影响因子：
4.3
通讯作者：
Li, Dequan
中科院分区：
文献类型：
--
作者：
Pan, Jiaowen;Zhang, Maoying;Li, Dequan
植物丝裂原激活蛋白激酶（MAPK）级联在一系列生物和非生物胁迫反应中发挥着关键作用。在这项研究中，我们从玉米（Zea maysL.）中分离出一个新的D组MAPK基因ZmMPK17。 ZmMPK17 主要定位于细胞核，其 C 端结构域延伸被认为对此至关重要。 Northern-blot分析表明ZmMPK17转录参与响应外源信号分子，如脱落酸、过氧化氢、水杨酸、茉莉酸和乙烯，并由低温和渗透胁迫诱导。过氧化氢和Ca2+介导PEG诱导的ZmMPK17转录水平下调，Ca2+还介导低温诱导的ZmMPK17表达。烟草中ZmMPK17的过度表达通过影响抗氧化防御系统在渗透胁迫下积累较少的活性氧。与对照植物相比，转基因烟草通过提高发芽率、提高脯氨酸和可溶性糖水平而表现出增强的耐冷性。在寒冷和渗透胁迫条件下，ZmMPK17过表达株系中NtERD10基因的转录水平高于对照植物。ZmMPK17过表达植物对病毒病原体的抵抗力增强，病程相关基因PR1的表达显着增加，表明ZmMPK17可能参与SA介导的病原体防御信号通路。
Plant mitogen-activated protein kinase (MAPK) cascades play a pivotal role in a range of biotic and abiotic stress responses. In this study, we isolated a novel group D MAPK gene,ZmMPK17, from maize (Zea maysL.). ZmMPK17 is localized mainly to the nucleus and its C-terminal domain extension is believed to be essential for this. Northern-blot analysis indicated thatZmMPK17transcription is involved in response to exogenous signaling molecules such as abscisic acid, hydrogen peroxide, salicylic acid, jasmonic acid and ethylene and induced by low temperature and osmotic stress. Hydrogen peroxide and Ca2+mediate PEG-induced downregulation ofZmMPK17at transcription level and Ca2+also mediates low temperature-induced expression ofZmMPK17.Overexpression ofZmMPK17in tobacco (Nicotoniatobaccum) accumulated less reactive oxygen species under osmotic stress by affecting antioxidant defense systems. Transgenic tobacco exhibited enhanced tolerance to cold by means of an increased germination rate, and increased proline and soluble sugar levels relative to control plants. The transcription levels ofNtERD10genes were higher inZmMPK17-overexpressing lines than in control plants under cold and osmotic stress conditions.ZmMPK17-overexpressing plants displayed enhanced resistance to viral pathogens, and the expression of the pathogenesis-related genePR1awas significantly increased, indicating that ZmMPK17 might be involved in SA-mediated pathogen defense-signaling pathways.