cDNA cloning and expressions of cytochrome P450 1A in zebrafish embryos.
cDNA cloning and expressions of cytochrome P450 1A in zebrafish embryos.
复制标题
DOI：
10.1292/jvms.64.829
复制
发表时间：
2002-09
期刊：
影响因子：
--
通讯作者：
K. Yamazaki;H. Teraoka;Wu Dong;J. Stegeman;T. Hiraga
中科院分区：
文献类型：
--
作者：
K. Yamazaki;H. Teraoka;Wu Dong;J. Stegeman;T. Hiraga
众所周知，细胞色素 P450 1A (CYP1A) 会被芳香烃诱导，包括 2,3,7,8-四氯二苯并-对二恶英 (TCDD)。我们确定了斑马鱼 (zfCYP1A) 中具有 5' 端和 3' 端的 CYP1A 开放阅读框的完整 cDNA 序列，这是一种有用的环境毒理学模型。 zfCYP1A 与哺乳动物、家禽和爪蟾 (51.9-60.4%) 以及其他鱼类 (63.8-89.2%) 的 CYP1A 具有很高的同一性。原位杂交和免疫组织化学显示，zfCYP1A在对照胚胎中几乎检测不到，但被TCDD显着诱导，尤其是在预孵化和孵化胚胎的心脏、血管内皮细胞、肠上皮、前肾和外珠被中。这些表达模式与 zfCYP1A 可能参与 TCDD 诱导的毒性一致。
Cytochrome P450 1A (CYP1A) is well known for being induced by aromatic hydrocarbons, including 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD). We determined the complete cDNA sequence of a CYP1A open reading frame with both 5'- and 3'-ends in zebrafish (zfCYP1A), a useful model for environmental toxicology. zfCYP1A shows high percentage identity with CYP1As of mammals, domestic fowl and xenopus (51.9-60.4%), as well as the other fish species (63.8-89.2%). As revealed by in situ hybridization and immunohistochemistry, zfCYP1A was scarcely detected in control embryos but was markedly induced by TCDD especially in heart, vascular endothelial cells, intestinal epithelium, pronephros and outer integument in both prehatched and hatched embryos. These expression patterns are consistent with possible involvement of zfCYP1A in TCDD-induced toxicities.