Regeneration in bipinnaria larvae of the bat star Patiria miniata induces rapid and broad new gene expression.
Regeneration in bipinnaria larvae of the bat star Patiria miniata induces rapid and broad new gene expression.
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蝙蝠星 Patiria miniata 的双翅果幼虫的再生诱导了快速而广泛的新基因表达。
DOI：
10.1016/j.mod.2016.08.003
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发表时间：
2016
影响因子：
2.6
通讯作者：
Wessel,Gary
中科院分区：
文献类型：
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作者：
Oulhen,Nathalie;Heyland,Andreas;Carrier,TylerJ;Zazueta-Novoa,Vanesa;Fresques,Tara;Laird,Jessica;Onorato,ThomasM;Janies,Daniel;Wessel,Gary
背景某些后生动物具有再生失去的身体部位的能力。成年人的这种现象在棘皮动物中有经典的描述，特别是在海星（海星总科）中。海星星星bipinnaria幼虫也可以快速有效地再生一个完整的幼虫后，手术二分法。从发育和生物医学的角度来看，了解扭转幼虫细胞命运的能力是很重要的;然而，棘皮动物再生的机制知之甚少。ResultsHere，我们描述了在蝙蝠starPatiria miniata平分后bipinnaria再生的过程。我们测试了在前半和后半的二羽藓中平分后的转录、翻译和细胞增殖活性以及SRAP的表达，SRAP被报道为海星星再生相关蛋白酶（Vickery et al.，2001年b）。此外，我们还发现了Vasa、dysferlin、vitellogenin 1和vitellogenin 2等基因的转录水平在分割后明显增加。结论分割后，尤其是前半部分，三个胚层的细胞命运发生了明显的变化。这些结果定义的分子事件，伴随着细胞命运的变化，同时在棘皮动物幼虫的再生反应。
BackgroundSome metazoa have the capacity to regenerate lost body parts. This phenomenon in adults has been classically described in echinoderms, especially in sea stars (Asteroidea). Sea star bipinnaria larvae can also rapidly and effectively regenerate a complete larva after surgical bisection. Understanding the capacity to reverse cell fates in the larva is important from both a developmental and biomedical perspective; yet, the mechanisms underlying regeneration in echinoderms are poorly understood.ResultsHere, we describe the process of bipinnaria regeneration after bisection in the bat starPatiria miniata. We tested transcriptional, translational, and cell proliferation activity after bisection in anterior and posterior bipinnaria halves as well as expression of SRAP, reported as a sea star regeneration associated protease (Vickery et al., 2001b). Moreover, we found several genes whose transcripts increased in abundance following bisection, including: Vasa, dysferlin, vitellogenin 1 and vitellogenin 2.ConclusionThese results show a transformation following bisection, especially in the anterior halves, of cell fate reassignment in all three germ layers, with clear and predictable changes. These results define molecular events that accompany the cell fate changes coincident to the regenerative response in echinoderm larvae.