Expression of CD14, CD16 and CD45RA on monocytes from periodontitis patients
Expression of CD14, CD16 and CD45RA on monocytes from periodontitis patients
复制标题
DOI：
10.1111/j.1600-0765.2004.00713.x
复制
发表时间：
2004-02-01
影响因子：
3.5
通讯作者：
Izumi, Y
中科院分区：
文献类型：
--
作者：
Nagasawa, T;Kobayashi, H;Izumi, Y
背景和目的：外周血单核细胞是一个异质性的群体，其表型在激活或分化时发生变化。大多数单核细胞强烈表达脂多糖（LPS）受体CD14，而不表达Fc γ受体III， CD16(CD14（++)CD16（-）单核细胞）。但在血透患者中，表达CD16且CD14减少的单核细胞(CD14（+)CD16（+）单核细胞）在炎症性疾病以及败血症和菌血症中增加。CD45RA在活化的单核细胞上表达，被认为是外周血单核细胞的激活标志物。本研究的目的是确定牙周炎患者单核细胞的表型和功能改变。方法：采集侵袭性牙周炎患者33例（女性22例，男性11例）、慢性牙周炎患者55例（女性35例，男性20例）和健康人群30例（女性16例，男性14例）外周血，采用流式细胞术检测CD14、CD16和CD45RA在单核细胞上的表达。用流式细胞术检测大肠杆菌和放线菌的LPS刺激CD16（+）和CD16（-）单核细胞产生白细胞介素6（IL-6）的情况。结果：慢性牙周炎患者CD14（+）和CD16（+）单核细胞比例明显升高。与健康受试者相比，侵袭性牙周炎患者表达CD45RA的单核细胞百分比显著增加。CD16（+）和CD16（-）单核细胞对来自大肠杆菌和放线菌comitans的LPS产生IL-6， CD16（+）单核细胞产生IL-6的细胞比例高于CD16（-）单核细胞，表明CD14(+)CD16（+）单核细胞代表一种高反应表型。结论：本研究表明，CD14(+)CD16（+）单核细胞和CD45RA（+）单核细胞在慢性和侵袭性牙周炎中分别升高。这些发现表明，可以通过监测单核细胞表面CD14、CD16和CD45RA的表达来评估牙周炎患者单核细胞的改变。
Background and objective: Peripheral blood monocytes are a heterogeneous population, with phenotypes that change on activation or differentiation. Most of the monocytes express lipopolysaccharide (LPS) receptor, CD14 intensely, and do not express Fc gamma receptor III, CD16 (CD14(++)CD16(-) monocytes). But monocytes expressing CD16 with reduced CD14 (CD14(+)CD16(+) monocytes) increase in inflammatory diseases as well as sepsis and bacteremia in hemodialysis patients. CD45RA is expressed on activated monocytes, and is regarded as an activation marker of peripheral blood monocytes. The purpose of this study was to determine the phenotypic and functional alteration of monocytes in periodontitis patients.Methods: Peripheral blood was collected from 33 aggressive periodontitis patients (22 females, 11 males), 55 chronic periodontitis patients (35 females, 20 males) and 30 healthy subjects (16 females, 14 males), and the expression of CD14, CD16 and CD45RA on monocytes was determined using flow cytometry. The production of interleukin-6 (IL-6) by CD16(+) and CD16(-) monocytes stimulated with LPS from Escherichia coli and Actinobacillus actinomycetemcomitans was also examined using flow cytometry.Results: The percentage of CD14(+)CD16(+) monocytes was significantly increased in chronic periodontitis patients. Percentage of monocytes expressing CD45RA was significantly increased in aggressive periodontitis patients compared to healthy subjects. CD16(+) and CD16(-) monocytes produced IL-6 in response to LPS from E. coli and A. actinomycetemcomitans, and the percentage of IL-6 producing cells was higher in CD16(+) monocytes than CD16(-) monocytes, suggesting that CD14(+)CD16(+) monocytes represent a hyper-reactive phenotype.Conclusions: The present study demonstrated that CD14(+)CD16(+) monocytes and CD45RA(+) monocytes were increased in chronic and aggressive periodontitis, respectively. These findings suggest that alteration of monocytes in periodontitis patients could be evaluated by monitoring the surface expression of CD14, CD16 and CD45RA on monocytes.