Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes
Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes
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DOI：
10.1016/j.febslet.2006.01.050
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发表时间：
2006-02-01
期刊：
影响因子：
3.5
通讯作者：
Holzbaur, ELF
中科院分区：
文献类型：
--
作者：
Ligon, LA;Shelly, SS;Holzbaur, ELF
微管加端蛋白CLIP-170和EB 1在体内动态跟踪生长的微管的尖端。在这里，我们研究这些蛋白质与微管在体外的协会。CLIP-170结合微管蛋白二聚体并共组装成生长的微管。EB 1结合微管蛋白二聚体更弱，因此没有观察到共组装。然而，EB 1结合CLIP-170，并与CLIP-170和微管蛋白形成共复合物，其被募集到生长的微管正末端。CLIP-170和EB 1之间的相互作用被相关的CAP-Gly蛋白p150（Glued）竞争性抑制，该蛋白在体内也定位于微管正末端。基于这些观察结果，我们提出了一个模型，在该模型中，不同的加端复合物的形成可能会有差异地影响体内微管动力学。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Microtubule plus-end proteins CLIP-170 and EB1 dynamically track the tips of growing microtubules in vivo. Here we examine the association of these proteins with microtubules in vitro. CLIP-170 binds tubulin dimers and co-assembles into growing microtubules. EB1 binds tubulin dimers more weakly, so no co-assembly is observed. However, EB1 binds to CLIP170, and forms a co-complex with CLIP-170 and tubulin that is recruited to growing microtubule plus ends. The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150(Glued), which also localizes to microtubule plus ends in vivo. Based on these observations, we propose a model in which the formation of distinct plus-end complexes may differentially affect microtubule dynamics in vivo. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.