Sex differences in adult zebrafish anxiolytic-like responses to diazepam and melatonin
Sex differences in adult zebrafish anxiolytic-like responses to diazepam and melatonin
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DOI：
10.1016/j.neulet.2019.134548
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发表时间：
2020-01-01
影响因子：
2.5
通讯作者：
Kaluefff, Allan V.
中科院分区：
文献类型：
--
作者：
Genario, Rafael;Giacomini, Ana C. V. V.;Kaluefff, Allan V.
性别差异是生物医学研究中的一个重要变量。斑马鱼(Danio Rerio)正在迅速成为翻译神经科学和神经药理学中一种重要的新型模式生物。在这里，我们在新型水槽测试中测试了成年斑马鱼在接触两种临床相关的常见抗焦虑药物安定和褪黑素后，性别对运动活动和类焦虑行为的影响。对照组雌性斑马鱼比雄性斑马鱼更活跃和焦虑，褪黑素(0.232 mg/L)对雌雄斑马鱼都有缓解焦虑的作用，但只有雄性斑马鱼对安定(16mgg/L)有反应。这项研究揭示了药理反应中的性别特异性，强调了性别差异在斑马鱼行为和药理分析中的重要性。这也可能与建立抗焦虑药物临床反应的性别差异模型有关。总的来说，我们的数据支持斑马鱼行为反应的性别差异，并加强了这种水生模型在中枢神经系统药物筛选中日益增长的实用性。
Sex differences are an important variable in biomedical research. The zebrafish (Danio rerio) is rapidly becoming a critical novel model organism in translational neuroscience and neuropharmacology. Here, we examine the effects of sex on locomotor activity and anxiety-like behavior in adult zebrafish tested in the novel tank test following their exposure to two clinically relevant, common anxiolytic drugs diazepam and melatonin. While control female zebrafish were more active and anxious than males, both sexes showed anxiolytic responses to melatonin (0.232 mg/L) but only males responded to diazepam (16 mu g/L). Revealing sex specificity in pharmacological responses, this study emphasizes the importance of sex differences in behavioral and pharmacological analyses in zebrafish. This may also be potentially relevant to modeling sex differences in clinical responses to anxiolytic drugs. Collectively, our data support sex differences in zebrafish behavioral responses and reinforce the growing utility of this aquatic model in CNS drug screening.