Glucocorticoid up-regulation of high-affinity interleukin 6 receptors on human epithelial cells.
Glucocorticoid up-regulation of high-affinity interleukin 6 receptors on human epithelial cells.
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糖皮质激素上调人上皮细胞上高亲和力白细胞介素 6 受体。
DOI：
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发表时间：
1990
影响因子：
11.1
通讯作者：
J. Content
中科院分区：
文献类型：
--
作者：
L. Snyers;L. de Wit;J. Content
白介素6(IL-6)是一种强大的多效性细胞因子，除其他外，可刺激B细胞产生免疫球蛋白和触发肝细胞急性期蛋白质合成。与IL-1类似，它是由炎症刺激后的单核细胞和巨噬细胞产生的。对人不同细胞系IL-6R表达的分析表明，地塞米松可上调一个人上皮细胞系(UAC)和两个人肝癌细胞系(HepG2和Hep3B)的IL-6R数量。使用代谢标记的IL-6的[35S]蛋氨酸和[35S]半胱氨酸的结合实验的Scatchard分析证实了这种作用。Northern印迹分析在基因表达水平上证实了这一点。这些结果为IL-6和糖皮质激素之间的联系提供了证据。它们可以代表一个系统的例子，在这个系统中，糖皮质激素的作用之一是更准确地定义细胞因子的靶标，它们可以至少部分解释经常观察到的IL-6和糖皮质激素之间的协同作用，特别是在肝细胞的情况下。
Interleukin 6 (IL-6) is a potent pleiotropic cytokine, known, among others, to stimulate immunoglobulin production by B cells and to trigger acute-phase protein synthesis by hepatocytes. Similar to IL-1, it is produced by monocytes and macrophages following an inflammatory challenge. Analysis of IL-6 receptor (IL-6R) expression on different human cell lines indicates that dexamethasone could up-regulate the number of IL-6R on one epithelial cell line (UAC) and on two hepatoma cell lines (HepG2 and Hep3B). This effect was confirmed by Scatchard analysis of binding experiments, using [35S]methionine and [35S]cysteine metabolically labeled IL-6. It was confirmed at the level of mRNA expression by Northern blot analysis. These results provide evidence for a link between IL-6 and glucocorticoids. They could represent an example of a system in which one role of glucocorticoids is to define more accurately the target of cytokines, and they could explain, at least partly, the frequently observed synergy between IL-6 and glucocorticoids, notably in the case of hepatocytes.
免疫细胞因子作用的分子基础。
影响因子：
2.7
作者：
Farrar,WL;Ferris,DK;Harel-Bellan,A
通讯作者：
Harel-Bellan,A