Redistribution of fodrin (a component of the cortical cytoplasm) accompanying capping of cell surface molecules.
Redistribution of fodrin (a component of the cortical cytoplasm) accompanying capping of cell surface molecules.
复制标题
胞质蛋白（皮质细胞质的一种成分）的重新分布伴随着细胞表面分子的加帽。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
M. Willard
中科院分区：
文献类型：
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作者：
J. Levine;M. Willard
Fodrin是一种由两个分子量分别为25万和24万的多肽组成的蛋白质，集中在神经元的皮质细胞质中，通过轴突运输过程向下移动。我们已经使用免疫荧光技术来确定当细胞表面配体通过交联剂被诱导重新分布时，Fodrin抗原是否也在非神经元细胞中移动。当3T3细胞与刀豆蛋白A和抗刀豆蛋白A共同孵育时，表面刀豆蛋白A受体形成聚集体，丝蛋白抗原形成相应的细胞内聚集体；(Ii)当B淋巴细胞与抗Ig共同孵育时，表面Ig形成帽，丝蛋白抗原形成细胞内亚帽；(3)当T淋巴细胞用抗H-2抗体处理后，H-2抗原形成帽，而丝蛋白形成相应的亚帽。这些观察表明，Fodrin抗原可以在非神经细胞内移动，也可以在轴突中移动，并且它们的组织可以通过表面蛋白和环境刺激之间的相互作用来调节。他们还提出了这样一种可能性，即Fodrin与在淋巴细胞中形成亚帽的其他蛋白质(例如，肌动蛋白、肌球蛋白和α-肌动蛋白)是负责帽形成过程的细胞机制的一个组成部分。我们认为，在淋巴细胞封顶和轴突运输中，Fodrin的运动之间的相似性是否表明这两个过程的某些方面是相关的。
Fodrin, a protein composed of two polypeptides with molecular weights of 250,000 and 240,000, is concentrated in the cortical cytoplasm of neurons, and moves down the axons by the process of axonal transport. We have used immunofluorescence techniques to determine whether fodrin antigens also move in non-neuronal cells when cell surface ligands are induced to redistribute by crosslinking them. A redistribution of fodrin antigens occurred in the following instances: (i) when 3T3 cells were incubated with concanavalin A and anti-concanavalin A, surface concanavalin A receptors formed aggregates and fodrin antigens formed corresponding intracellular aggregates; (ii) when B lymphocytes were incubated with anti-Ig, the surface Ig formed caps and fodrin antigens formed intracellular subcaps; (iii) when T lymphocytes were treated with anti-H-2 followed by a secondary antibody, the H-2 antigen formed caps and fodrin formed corresponding subcaps. These observations show that fodrin antigens can move within non-neuronal cells, as well as in axons, and that their organization can be regulated by interaction between surface proteins and environmental stimuli. They also raise the possibility that fodrin, together with other proteins that form subcaps in lymphocytes (e.g., actin, myosin, and alpha-actinin) is a component of the cellular machinery responsible for the capping process. We consider whether the similarities between the movements of fodrin in lymphocyte capping and axonal transport may indicate that certain aspects of these two processes are related.