Composite Hydrogels with Engineered Microdomains for Optical Glucose Sensing at Low Oxygen Conditions.
Composite Hydrogels with Engineered Microdomains for Optical Glucose Sensing at Low Oxygen Conditions.
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DOI：
10.3390/bios7010008
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发表时间：
2017-01-22
期刊：
影响因子：
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通讯作者：
McShane MJ
中科院分区：
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作者：
Bornhoeft LR;Biswas A;McShane MJ
人们越来越需要先进的工具，以实现对精确医学生物标记物的频繁监测。在这项工作中，我们提出了一种基于复合水凝胶的系统，提供实时光学生物分析物监测。响应材料是海藻酸盐(ANA)，由嵌入生物活性纳米膜的磷光微域的海藻酸盐水凝胶组成；钯四甲基苯基卟啉作为光学指示剂，葡萄糖氧化酶作为模型酶，以及逐层沉积的聚电解质多层膜(PEM)作为扩散屏障。戊二醛交联型纳米膜显著减少了葡萄糖的扩散(179%)，而氧的转运没有受到明显影响。评估了ANA水凝胶在环境和生理氧气水平下对葡萄糖阶跃变化的响应，揭示了灵敏度和动态范围的受控调节。通过交替将响应性的ANA水凝胶暴露于极高的葡萄糖浓度和零葡萄糖浓度来评估稳定性，结果表明，在20个周期中，响应没有显著差异。这些ANA水凝胶代表了一种基于生物兼容材料的生物传感的有吸引力的方法，这种材料可以用作能够进行光学询问的微创、可植入的设备。本研究中研究的葡萄糖响应性复合材料模型将作为模板，可被翻译为检测额外的分析物(例如，乳酸、尿素、丙酮酸、胆固醇)，并可用于监测其他慢性疾病。
There is a growing need for advanced tools that enable frequent monitoring of biomarkers for precision medicine. In this work, we present a composite hydrogel-based system providing real-time optical bioanalyte monitoring. The responsive material, alginate-in-alginate (AnA), is comprised of an alginate hydrogel with embedded bioactive, nanofilm-coated phosphorescent microdomains; palladium tetracarboxyphenylporphyrin serves as an optical indicator, glucose oxidase as a model enzyme, and layer-by-layer deposited polyelectrolyte multilayers (PEMs) as the diffusion barrier. Glutaraldehyde crosslinking of the nanofilms resulted in a dramatic reduction in glucose diffusion (179%) while oxygen transport was not significantly affected. The responses of the AnA hydrogels to step changes of glucose at both ambient and physiological oxygen levels were evaluated, revealing controlled tuning of sensitivity and dynamic range. Stability, assessed by alternately exposing the responsive AnA hydrogels to extremely high and zero glucose concentrations, resulted in no significant difference in the response over 20 cycles. These AnA hydrogels represent an attractive approach to biosensing based on biocompatible materials that may be used as minimally-invasive, implantable devices capable of optical interrogation. The model glucose-responsive composite material studied in this work will serve as a template that can be translated for sensing additional analytes (e.g., lactate, urea, pyruvate, cholesterol) and can be used for monitoring other chronic conditions.