Variations in fecal Schistosoma japonicum egg counts
Variations in fecal Schistosoma japonicum egg counts
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DOI：
10.4269/ajtmh.1998.59.370
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发表时间：
1998-09-01
影响因子：
3.3
通讯作者：
Gryseels, B
中科院分区：
文献类型：
--
作者：
Yu, J;De Vlas, SJ;Gryseels, B
对中国江西省朱溪行政村粪便日本血吸虫虫卵计数的变化进行了研究。连续 7 天使用重复的标准 41.5 毫克 Kato-Katz 厚涂片收集了来自两个自然（个体）村庄（高流行村 I 和低流行村 II）的 570 名个体的群体粪便检查。村 I（n = 356）中至少有一次阳性计数的个体比例从单次测量后的 42.4% 增加到 7 次测量后的 68.3%，村 II（n = 214）从 17.0% 增加到 36.0%。这表明重复的日本血吸虫卵计数变化非常大，并且 Kato-Katz 技术相当缺乏灵敏度；通过一次或几次测量尤其可以漏掉轻度和中度感染。观察到的单个卵子计数的日常变化是高度聚合的（方差高于平均值）并暗示负二项式分布。三天内对五个人的粪便标本的不同位置进行重复采样，显示出明显的趋势：从粪便的开始到结束、从外层到中心，虫卵数量逐渐减少。来自 44 名阳性个体的粪便样本的特定部分（10-30 克）的 10 个多个样本仍然显示卵数聚集，特别是对于高强度感染。这意味着每日重复的日本血吸虫虫卵计数的聚集不能单独用特定的日常成分和粪便中不同位置虫卵浓度的变化来解释。粪便部分内还存在虫卵聚集。
Variations in fecal Schistosoma japonicum egg counts were studied in ZhuXi administrative village, JiangXi Province, China. Population stool examinations were collected with duplicate, standard, 41.5-mg Kato-Katz thick smears on seven consecutive days for 570 individuals from two natural (individual) villages: village I with high endemicity and village II with low endemicity. The proportion of individuals with at least one positive count increased from 42.4% after a single measurement to 68.3% after seven measurements in village I (n = 356), and from 17.0% to 36.0% in village II (n = 214), respectively. This demonstrates a very high variation in repeated S, japonicum egg counts and a considerable lack of sensitivity of the Kato-Katz technique; light and moderate infections are especially missed with a single or a few measurements. The observed day-to-day variation in individual egg counts is highly aggregated (variance higher than the mean) and suggestive of a negative binomial distribution. For five individuals on three days, repeated sampling from different locations of a stool specimen shows a clear trend with egg counts decreasing from the beginning of the stool to the end and from the outside layer to the center. Ten multiple samples from a particular subsection (10-30 g) of a stool specimen for 44 positive individuals still showed aggregation in egg counts, particularly for high intensities of infection. This means that the aggregation in repeated daily S. japonicum egg counts cannot be explained alone by a specific day-to-day component and variation in the concentration of eggs at different locations in the stool. There also exists clustering of eggs within parts of the stool.