Kinins are generated in vivo following nasal airway challenge of allergic individuals with allergen.
Kinins are generated in vivo following nasal airway challenge of allergic individuals with allergen.
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激肽是在过敏个体的鼻气道受到过敏原刺激后在体内产生的。
DOI：
10.1172/jci111127
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Lichtenstein,LM
中科院分区：
文献类型：
--
作者：
Proud,D;Togias,A;Naclerio,RM;Crush,SA;Norman,PS;Lichtenstein,LM
使用最近开发的鼻腔挑战模型，我们获得了第一次清楚地证明在活体局部过敏反应中激动素产生的数据。过敏者(n=8)和匹配的非过敏性对照组(n=8)用适当的抗原进行鼻腔攻击，并在攻击前后进行鼻腔冲洗。洗涤后测定激动素、组胺和[~3H]-N-α-对甲苯磺酰-L-精氨酸甲酯(TAME)-酯酶活性。放射免疫分析(RIA)发现，所有变态反应患者(5,560+/-1,670 pg/ml)鼻液中激动素的生成均增加，而对照组(38+/-16 pg/ml)则未见。激肽的存在与组胺、驯化酯酶活性及临床症状的出现高度相关(P<0.001)。连续稀释的鼻洗液产生与标准曲线平行的RIA位移曲线，标准激动素的回收率为100+/-4%(n=14)。激动素的恢复在变态反应组和对照组中是相同的，并且不随抗原攻击而有显著差异。鼻腔冲洗液中的激动素免疫反应稳定，不被乙醇沉淀，但被羧基肽酶B完全破坏，均匀分布在鼻腔冲洗液的溶胶相和凝胶相之间。对鼻液中免疫活性激动素的高效液相分析表明，它是赖氨酸激动素和缓激肽的混合物。我们得出结论，激肽是在鼻子的局部过敏反应中产生的，可能有助于过敏反应的症状。
Using a recently developed model of nasal challenge, we have obtained data that clearly demonstrate, for the first time, kinin generation during a local allergic reaction in vivo. Allergic individuals (n = 8) and matched nonallergic controls (n = 8) were challenged intranasally with the appropriate antigen and nasal washes were taken before and after challenge. Washes were assayed for kinin, histamine, and [3H]-N-alpha-tosyl-L-arginine methyl ester (TAME)-esterase activity. Increased kinin generation was found by radioimmunoassay (RIA) in the nasal washes of all the allergics (5,560 +/- 1,670 pg/ml) but in none of the controls (38 +/- 16 pg/ml). The presence of kinin was highly correlated with that of histamine and TAME-esterase activity and with the onset of clinical symptoms (P less than 0.001). Serial dilutions of nasal washes produced RIA displacement curves that paralleled the standard curve, and recovery of standard kinins that were added to nasal washes was 100 +/- 4% (n = 14). Kinin recovery was identical in both allergics and controls and did not vary significantly with antigen challenge. The immunoreactive kinin in nasal washes was stable to boiling and not precipitated by ethanol, but completely destroyed by carboxypeptidase B. It was evenly distributed between the sol and gel phases of nasal washes. High performance liquid chromatography analysis of the immunoreactive kinin in nasal washes showed it to be a mixture of lysylbradykinin and bradykinin. We conclude that kinins are produced during local allergic reactions in the nose and may contribute to the symptomatology of the allergic response.
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