The Wilms' tumor gene WT1-GFP knock-in mouse reveals the dynamic regulation of WT1 expression in normal and leukemic hematopoiesis
The Wilms' tumor gene WT1-GFP knock-in mouse reveals the dynamic regulation of WT1 expression in normal and leukemic hematopoiesis
复制标题
DOI：
10.1038/sj.leu.2404752
复制
发表时间：
2007-08-01
期刊：
影响因子：
11.4
通讯作者：
Weissman, I. L.
中科院分区：
文献类型：
--
作者：
Hosen, N.;Shirakata, T.;Weissman, I. L.
Wilms'肿瘤基因WT 1在大多数人类白血病中过表达，而与疾病亚型无关。为了表征WT 1在正常和肿瘤性造血过程中的表达模式，我们产生了敲入报告基因绿色荧光蛋白（GFP）小鼠（WT 1（GFP/+）），并测定了WT 1在正常和白血病造血细胞中的表达。在正常的造血细胞中，WT 1在长期（LT）造血干细胞（HSC）中无表达，
The Wilms' tumor gene WT1 is overexpressed in most of human leukemias regardless of disease subtypes. To characterize the expression pattern of WT1 during normal and neoplastic hematopoiesis, we generated a knock-in reporter green fluorescent protein (GFP) mouse (WT1(GFP/+)) and assayed for WT1 expression in normal and leukemic hematopoietic cells. In normal hematopoietic cells, WT1 was expressed in none of the long-term (LT) hematopoietic stem cells (HSC) and very few (