Microfluidics-based diagnostics of infectious diseases in the developing world
Microfluidics-based diagnostics of infectious diseases in the developing world
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DOI：
10.1038/nm.2408
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发表时间：
2011-08-01
期刊：
影响因子：
82.9
通讯作者：
Sia, Samuel K.
中科院分区：
文献类型：
--
作者：
Chin, Curtis D.;Laksanasopin, Tassaneewan;Sia, Samuel K.
当今科学和工程领域面临的最大挑战之一是开发技术，以改善世界上最贫困地区人民的健康。在这里，我们将微流体中的制造、流体处理和信号检测的新程序集成到一个简单易用的即时检测（POC）分析中，该分析忠实地复制了ELISA的所有步骤，并降低了总材料成本。我们在卢旺达对数百个当地收集的人类样本进行了这种“mChip”检测。该芯片在仅使用1 μ l未处理的全血的HIV诊断中具有优异的性能，并且能够同时诊断HIV和梅毒，其灵敏度和特异性与参考台式测定法的灵敏度和特异性相媲美。与目前大多数快速测试不同，mChip测试不需要用户解释信号。总的来说，我们展示了一种使用微流体和纳米颗粒来实现复杂实验室检测的综合策略，以实现POC诊断和远程环境中传染病的早期检测。
One of the great challenges in science and engineering today is to develop technologies to improve the health of people in the poorest regions of the world. Here we integrated new procedures for manufacturing, fluid handling and signal detection in microfluidics into a single, easy-to-use point-of-care (POC) assay that faithfully replicates all steps of ELISA, at a lower total material cost. We performed this 'mChip' assay in Rwanda on hundreds of locally collected human samples. The chip had excellent performance in the diagnosis of HIV using only 1 mu l of unprocessed whole blood and an ability to simultaneously diagnose HIV and syphilis with sensitivities and specificities that rival those of reference benchtop assays. Unlike most current rapid tests, the mChip test does not require user interpretation of the signal. Overall, we demonstrate an integrated strategy for miniaturizing complex laboratory assays using microfluidics and nanoparticles to enable POC diagnostics and early detection of infectious diseases in remote settings.