CROP/Luc7A, a novel serine/arginine-rich nuclear protein, isolated from cisplatin-resistant cell line
CROP/Luc7A, a novel serine/arginine-rich nuclear protein, isolated from cisplatin-resistant cell line
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DOI：
10.1016/s0014-5793(99)01744-5
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发表时间：
2000-01-14
期刊：
影响因子：
3.5
通讯作者：
Ueda, K
中科院分区：
文献类型：
--
作者：
Nishii, Y;Morishima, M;Ueda, K
利用差异显示技术从顺铂耐药细胞系中克隆了一种新的SR蛋白，命名为顺铂耐药相关过表达蛋白（CROP）。CROP的432个氨基酸的N-半部分含有半胱氨酸/组氨酸基序和亮氨酸拉链样重复序列。C-半主要由带电和极性氨基酸组成：精氨酸（58个残基或25%），谷氨酸（36个残基或16%），丝氨酸（35个残基或15%），赖氨酸（30个残基，13%）和天冬氨酸C-半是极其亲水的并且包含富含赖氨酸和谷氨酸残基的结构域，富含交替的精氨酸和谷氨酸残基，并且富含精氨酸和丝氨酸残基。富含精氨酸/丝氨酸的结构域由一系列8个氨基酸的不完全重复基序（共有序列，Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg）主导，其已在RNA剪接因子中发现。RNA酶保护试验和蛋白质印迹分析表明，在顺铂抗性ACHN/CDDP细胞中，CROP的表达在mRNA和蛋白质水平上比宿主ACHN细胞高约2-3倍。CROP是酵母Luc 7 p的人类同源物，它应该参与5 '-剪接位点识别，并且对于营养生长至关重要。(C)2000年欧洲生物化学学会联合会。
A novel putative SR protein, designated cisplatin resistance-associated overexpressed protein (CROP), has been cloned from cisplatin-resistant cell lines by differential display. The N-half of the deduced amino acid sequence of 432 amino acids of CROP contains cysteine/histidine motifs and leucine zipper-like repeats. The C-half consists mostly of charged and polar amino acids: arginine (58 residues or 25%), glutamate (36 residues or 16%), serine (35 residues or 15%), lysine (30 residues, 13%), and aspartate (20 residues or 9%), The C-half is extremely hydrophilic and comprises domains rich in lysine and glutamate residues, rich in alternating arginine and glutamate residues, and rich in arginine and serine residues. The arginine/serine-rich domain is dominated by a series of 8 amino acid imperfect repetitive motif (consensus sequence, Ser-Arg-Ser-Arg-Asp/Glu-Arg-Arg-Arg), which has been found in RNA splicing factors. The RNase protection assay and Western blotting analysis indicate that the expression of CROP is about 2-3-fold higher in mRNA and protein levels in cisplatin-resistant ACHN/CDDP cells than in host ACHN cells. CROP is the human homologue of yeast Luc7p, which is supposed to be involved in 5'-splice site recognition and is essential for vegetative growth. (C) 2000 Federation of European Biochemical Societies.