Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle
Nek2 localizes to multiple sites in mitotic cells, suggesting its involvement in multiple cellular functions during the cell cycle
复制标题
DOI：
10.1006/bbrc.2001.6212
复制
发表时间：
2002-01-18
影响因子：
3.1
通讯作者：
Rhee, K
中科院分区：
文献类型：
--
作者：
Kim, YH;Choi, JY;Rhee, K
Nek2是一种哺乳动物蛋白激酶，其在结构上与构巢曲霉中的有丝分裂调节因子NIMA同源。为了了解Nek 2在细胞周期中可能参与的细胞过程，我们研究了Nek 2在有丝分裂细胞中的表达和亚细胞定位。在培养的细胞中，观察到Nek2蛋白水平以细胞周期阶段特异性方式进行调节。Nek2表达的细胞周期阶段特异性也在体内经历有丝分裂的细胞中得到证实。Nek2蛋白定位于细胞核和细胞质中的整个细胞周期，但表现出动态变化的分布，这取决于细胞周期阶段。Nek 2在前期至中期与染色体结合，进入后期后解离。Nek2在细胞分裂末期出现在胞质桥的中段。Nek2也与中心体在整个细胞周期，如以前观察到的其他人。此外，Nek 2的核定位在S期增加。Nek2的这种动态行为表明，Nek2可能是一个有丝分裂调节因子，参与不同的细胞周期事件。(C)2002年，Elsevier Science。
Nek2 is a mammalian protein kinase that is structurally homologous to NIMA, a mitotic regulator in Aspergillus nidulans. To understand the possible cellular processes in which Nek2 participates during the cell cycle, we investigated the expression and subcellular localization of Nek2 in mitotic cells. The Nek2 protein levels were observed to be regulated in a cell cycle stage-specific manner in cultured cells. The cell cycle stage specificity of Nek2 expression was also confirmed in cells undergoing mitosis in vivo. Nek2 proteins were localized in both the nucleus and cytoplasm throughout the cell cycle, but exhibited dynamic changes in distribution, depending on the cell cycle stage. Nek2 was associated with chromosomes from prophase to metaphase and then was dissociated upon entering into anaphase. Nek2 then appeared at the midbody of the cytoplasmic bridge at telophase. Nek2 was also associated with the centrosome throughout the cell cycle as observed previously by others. Additionally, the nuclear localization of Nek2 was increased during S phase. Such dynamic behavior of Nek2 suggests that Nek2 may be a mitotic regulator that is involved in diverse cell cycle events. (C) 2002 Elsevier Science.