Kainate-induced K+ efflux and plasma membrane depolarization in cultured cerebellar granule cells.
Kainate-induced K+ efflux and plasma membrane depolarization in cultured cerebellar granule cells.
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培养的小脑颗粒细胞中红藻氨酸诱导的 K 流出和质膜去极化。
DOI：
10.1097/00001756-200101220-00020
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发表时间：
2001
期刊：
影响因子：
1.7
通讯作者：
Mienville,JM
中科院分区：
文献类型：
--
作者：
Kiedrowski,L;Mienville,JM
越来越多的证据表明，离子亲性谷氨酸受体的激活导致胞浆[K+]([K+]C)的显著变化，[K+]C是质膜(PM)电位的主要决定因素。由于Em影响钙离子等关键阳离子的通量，因此准确地定量谷氨酸受体激动剂作用下神经元的[K+]C和Em是非常重要的。本文研究了小脑颗粒细胞原代培养中[K+]C与Em的关系，发现红藻氨酸使[K+]C在10 mM以下迅速下降。在含有10和150mMK+的贴片电极上，我们测定了红藻氨酸分别使PM去极化到−2或−28 mV。因此，红藻氨酸实际引起的PM去极化比常规的富K+电极测量的PM去极化大得多。
It is becoming increasingly evident that activation of ionotropic glutamate receptors leads to significant changes in cytosolic [K+]([K+] C), a major determinant of the plasma membrane (PM) potential, Em. Since E m affects fluxes of key cations, such as Ca 2+, it is important to precisely quantify [K+] C and E m in neurons exposed to glutamate receptor agonists. Here we studied the relationships between [K+] C and E m in primary cultures of cerebellar granule cells, and found that kainate elicits a rapid drop in [K+] C below 10 mM. Using patch electrodes containing 10 or 150 mM K+, we determined that kainate depolarizes the PM to− 2 or− 28 mV, respectively. Therefore, the actual PM depolarization elicited by kainate is much larger than that routinely measured with K+-rich electrodes.