miRNA-dependent translational repression in the Drosophila ovary.
miRNA-dependent translational repression in the Drosophila ovary.
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DOI：
10.1371/journal.pone.0004669
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Macdonald PM
中科院分区：
文献类型：
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作者：
Reich J;Snee MJ;Macdonald PM
果蝇卵巢是一个富含基因表达转录后调控的组织。许多调节因子是通过遗传筛选鉴定的蛋白质。最近发现的microRNA在其他动物和组织中似乎调节了所有mRNA中很大一部分的翻译，提出了它们也可能在卵子发生过程中起作用的可能性。然而，在卵巢中没有直接证明microRNA依赖的翻译抑制。在此，对具有或不具有合成miR-312结合位点的转基因的转录物和蛋白质水平的定量分析显示，结合位点确实赋予翻译抑制。这种效应取决于细胞产生microRNA的能力。通过与其他细胞类型中的microRNA依赖性翻译抑制相比，预期调节的mRNA和介导抑制的蛋白质因子在海绵体中富集，海绵体是与其他细胞中发现的P体具有广泛相似性的亚细胞结构。然而，没有观察到这种富集。我们的研究结果揭示了果蝇卵巢中的各种转录后调控机制，并对卵巢中使用的miRNA依赖的翻译控制机制产生了影响。
The Drosophila ovary is a tissue rich in post-transcriptional regulation of gene expression. Many of the regulatory factors are proteins identified via genetic screens. The more recent discovery of microRNAs, which in other animals and tissues appear to regulate translation of a large fraction of all mRNAs, raised the possibility that they too might act during oogenesis. However, there has been no direct demonstration of microRNA-dependent translational repression in the ovary. Here, quantitative analyses of transcript and protein levels of transgenes with or without synthetic miR-312 binding sites show that the binding sites do confer translational repression. This effect is dependent on the ability of the cells to produce microRNAs. By comparison with microRNA-dependent translational repression in other cell types, the regulated mRNAs and the protein factors that mediate repression were expected to be enriched in sponge bodies, subcellular structures with extensive similarities to the P bodies found in other cells. However, no such enrichment was observed. Our results reveal the variety of post-transcriptional regulatory mechanisms that operate in the Drosophila ovary, and have implications for the mechanisms of miRNA-dependent translational control used in the ovary.
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影响因子：
9.2
作者：
Forrest, KM;Gavis, ER
通讯作者：
Gavis, ER
影响因子：
64.5
作者：
Kiriakidou, Marianthi;Tan, Grace S.;Mourelatos, Zissimos
通讯作者：
Mourelatos, Zissimos
影响因子：
9.2
作者：
Chen, Yu;Pane, Attilio;Schupbach, Trudi
通讯作者：
Schupbach, Trudi
DOI：
10.1073/pnas.0506482102
发表时间：
2005-11-22
影响因子：
11.1
作者：
Humphreys, DT;Westman, BJ;Preiss, T
通讯作者：
Preiss, T
影响因子：
4.4
作者：
通讯作者：
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