Advances in the determination of nucleic acid conformational ensembles.
Advances in the determination of nucleic acid conformational ensembles.
复制标题
DOI：
10.1146/annurev-physchem-040412-110059
复制
发表时间：
2014
影响因子：
14.7
通讯作者：
Al-Hashimi HM
中科院分区：
文献类型：
--
作者：
Salmon L;Yang S;Al-Hashimi HM
核酸的构象变化在遗传信息在活细胞中的储存、转移和处理中起着关键作用。在这里，我们描述了新的方法，采用了广泛的实验数据，包括NMR衍生的化学位移和残余偶极耦合，小角X射线散射，和计算方法，如分子动力学模拟，以确定合奏的DNA和RNA在原子分辨率。我们审查的互补信息，可以从不同的数据集和各种方法，已开发的联合收割机这些数据与计算方法相结合，构建合奏和评估其不确定性。最后，我们调查使用这些方法确定的RNA和DNA合奏，突出了迄今为止已经获得的独特的物理和功能的见解。
Conformational changes in nucleic acids play a key role in the way genetic information is stored, transferred, and processed in living cells. Here, we describe new approaches that employ a broad range of experimental data, including NMR derived chemical shifts and residual dipolar couplings, small angle X-ray scattering, and computational approaches such as molecular dynamics simulations, to determine ensembles of DNA and RNA at atomic resolution. We review the complimentary information that can be obtained from diverse sets of data and the various methods that have been developed to combine these data with computational methods to construct ensembles and assess their uncertainty. We conclude by surveying RNA and DNA ensembles determined using these methods, highlighting the unique physical and functional insights that have been obtained so far.