New developments in hematopoietic stem cell expansion
New developments in hematopoietic stem cell expansion
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造血干细胞扩增的新进展
DOI：
10.1097/00062752-199801000-00013
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发表时间：
1998
影响因子：
3.2
通讯作者：
R. Henschler
中科院分区：
文献类型：
--
作者：
C. von Kalle;H. Glimm;G. Schulz;R. Mertelsmann;R. Henschler
在离体培养中维持、操作和扩增人类造血干细胞的可能性有助于为患者提供纯度和性能得到改善的自体和同种异体干细胞移植，并可以提供造血系统基因治疗的机会。未成熟人类造血祖细胞和人类造血干细胞离体培养的最新进展为离体培养中造血干细胞的定性和定量维持以及可能的数量扩展提供了实验证据，使离体移植工程成为现实的可能性。这篇综述总结了该领域的最新进展、其监管意义及其在造血基因治疗中的应用。
The possibility of maintaining, manipulating, and expanding human hematopoietic stem cells in ex vivo culture could help to provide patients with autologous and allogeneic stem cell transplants improved in purity and perfomance and could offer access to gene therapy of the hematopoietic system. Recent advances in the ex vivo culture of immature human hematopoietic progenitor cells and human hematopoietic stem cells have led to experimental evidence for the qualitative and quantitative maintenance and possible numerical expansion of hematopoietic stem cells in ex vivo culture, making ex vivo graft engineering a realistic possibility. This review summarizes recent developments in the field, their regulatory implications and their application in hematopoietic gene therapy.