Infectious alphavirus production from a simple plasmid transfection+.
Infectious alphavirus production from a simple plasmid transfection+.
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DOI：
10.1186/1743-422x-8-356
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发表时间：
2011-07-19
期刊：
影响因子：
4.8
通讯作者：
Geiss BJ
中科院分区：
文献类型：
--
作者：
Steel JJ;Henderson BR;Lama SB;Olson KE;Geiss BJ
We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the production of infectious virus. Transfection of as little as 125 ng of plasmid is able to produce 1 × 108 plaque forming units/ml (PFU/ml) of infectious virus 48 hours post-transfection. This system represents a more efficient method for producing recombinant Sindbis viruses.
