Differential expression of angiogenic and vasodilatory factors by invasive trophoblast giant cells depending on depth of invasion
Differential expression of angiogenic and vasodilatory factors by invasive trophoblast giant cells depending on depth of invasion
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DOI：
10.1002/dvdy.10291
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发表时间：
2003-06-01
影响因子：
2.5
通讯作者：
Cross, JC
中科院分区：
文献类型：
--
作者：
Hemberger, M;Nozaki, T;Cross, JC
妊娠期子宫床发生显著变化，包括子宫基质的增殖和蜕膜化以及母体血管的重塑和血管生成。胎儿来源的滋养层巨细胞侵入子宫，在那里它们进入母体血液循环，以确保胚胎有足够的营养供应。在妊娠早期至中期的连续切片中，我们确定了滋养层浸润的确切距离以及可能影响母体循环系统重塑和重定向的血管生成、血管扩张和抗凝因子的表达。滋养层衍生物在离胎盘边缘大约300 μ m的距离处被检测到，在那里它们完全沿着沿着蜕膜的中线分布。侵袭最远的细胞特征性地表达增殖素和增殖素相关蛋白，这些激素影响内皮细胞迁移和血管形成。偶尔，这些细胞取代正常的血管内皮细胞，并获得了“假内皮”的形状。在胎盘边界外50-80 μ m处观察到完全的血管崩解，其中母体血液完全由滋养层巨细胞衍生的血窦网络内衬。这种血腔衬里的转变与滋养层表达各种血管舒张和抗凝因子相关，这些因子可能促进血液流向胎盘。对畸胎瘤样肿瘤的分析表明，滋养层巨细胞诱导的血流促进和重定向并不局限于子宫环境。这些结果表明，滋养层巨细胞具有吸引和增加血流的内在能力，并逐渐取代血管内皮，导致形成完全由滋养层细胞内衬的管道。发展动力学227.185-191，2003年。(C)2003 Wiley-Liss，Inc.
The uterine bed undergoes remarkable changes during pregnancy, including proliferation and decidualization of the uterine stroma and remodeling and anglogenesis of the maternal vasculature. Fetal-derived trophoblast giant cells invade into the uterus where they gain access to the maternal blood circulation to ensure sufficient nutrient supply of the embryo. In serial sections through early- to mid-gestation conceptuses, we have determined the exact distance of trophoblast invasion and the expression of angiogenic, vasodilatory, and anticoagulative factors that are likely to influence remodeling and redirection of the maternal circulatory system. Trophoblast derivatives were detected at a distance as far as similar to300 mum from the placental border, where they are allocated exclusively along the mid-line of the decidua. The farthest invading cells characteristically expressed proliferin and proliferin-related protein, hormones that affect endothelial cell migration and vascularization. Occasionally, these cells replaced the normal vascular endothelium and acquired a "pseudo-endothelial" shape. Complete vascular disintegration was observed 50-80 mum outside of the placental border where maternal blood was entirely lined by a trophoblast giant cell-derived network of blood sinuses. This transition in blood space lining correlated with trophoblast expression of various vasodilatory and anticoagulative factors that are likely to promote blood flow toward the placenta. Analysis of teratocarcinomalike tumors demonstrated that trophoblast giant cell-induced promotion and redirection of blood flow is not restricted to the uterine environment. These results show that trophoblast giant cells have the intrinsic capacity to attract and increase blood flow and to gradually displace the vascular endothelium resulting in the formation of canals entirely lined by trophoblast cells. Developmental Dynamics 227.185-191, 2003. (C) 2003 Wiley-Liss, Inc.