The Minimal Arsenic Concentration Required to Inhibit the Activity of Thyroid Peroxidase Activity In Vitro
The Minimal Arsenic Concentration Required to Inhibit the Activity of Thyroid Peroxidase Activity In Vitro
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DOI：
10.1007/s12011-008-8183-y
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发表时间：
2008-12-01
影响因子：
3.9
通讯作者：
Jansen, Kevin P.
中科院分区：
文献类型：
--
作者：
Palazzolo, Dominic L.;Jansen, Kevin P.
已知砷化合物会干扰正常的甲状腺功能。因此，我们设计了一个实验，以确定在体外抑制甲状腺过氧化物酶（TPO）活性所需的三氧化二砷（As 2 O3）的最低浓度。在不存在（对照）或存在砷（0.1、1.0、5.0和10 ppm）的情况下，在10分钟孵育期内，通过荧光光度法测定商业制备的人TPO的活性。这项研究的结果表明，一个显着的剂量-反应关系，最高浓度的砷产生最大量的TPO抑制。与对照组相比，0.1 ppm砷对TPO活性没有影响。孵育2分钟，在1.0，5.0，或10 ppm砷的存在下抑制TPO活性的4%，9%，和9%的控制，分别。在1.0或5.0 ppm砷的存在下孵育10分钟后，TPO活性分别恢复到对照的92%和54%，而10 ppm砷的存在进一步抑制TPO活性至对照的1%。总之，三氧化二砷以剂量依赖性方式抑制体外TPO活性，抑制该活性所需的最小剂量为0.1至1 ppm。
Arsenical compounds are known to interfere with normal thyroid function. Therefore, we designed an experiment to determine the minimal concentration of arsenic trioxide (As2O3) required to inhibit thyroid peroxidase (TPO) activity in vitro. The activity of commercially prepared human TPO was assayed spectrophotometrically in the absence (control) or presence of arsenic (0.1, 1.0, 5.0, and 10 ppm) during a 10-min incubation period. The results of this study indicate a significant dose-response relationship with the highest concentration of arsenic producing the greatest amount of TPO inhibition. Compared to controls, 0.1 ppm arsenic had no effect on TPO activity. Incubation for 2 min in the presence of 1.0, 5.0, or 10 ppm arsenic inhibited TPO activity to 4%, 9%, and 9% of control, respectively. After 10 min incubation in the presence of 1.0 or 5.0 ppm arsenic, TPO activity returned to 92% and 54% of control, respectively, while the presence of 10 ppm arsenic further inhibited TPO activity to 1% of control. In summary, arsenic trioxide inhibits in vitro TPO activity in a dose-dependent manner, and the minimal dose required to inhibit this activity is between 0.1 and 1 ppm.