A lysosomal probe for monitoring of pH in living cells and ovarian tumour
A lysosomal probe for monitoring of pH in living cells and ovarian tumour
复制标题
用于监测活细胞和卵巢肿瘤 pH 值的溶酶体探针
DOI：
10.1016/j.dyepig.2016.12.043
复制
发表时间：
2017
影响因子：
4.5
通讯作者：
Shiguo Sun
中科院分区：
文献类型：
--
作者：
Bo Li;Guang bo Ge;Lanlan Wen;Ye Yuan;Ran Zhang;Xiaojun Peng;Fengyu Liu;Shiguo Sun
细胞内pH值对于调节各种细胞行为和病理状况很重要。我们合成了一种基于罗丹明的溶酶体 pH 探针（以下简称 RD），pKa 为 4.10。该探针在pH 3.25至7.20之间表现出良好的“开关”可逆性，持续至少5个循环。RD可根据不同的荧光响应区分癌细胞与正常细胞。RD在MCF-7细胞中青蒿琥酯、氯喹和地塞米松诱导的溶酶体pH值的检测中表现良好。卵巢肿瘤中RD的强烈荧光表明其酸性较高 肿瘤组织中的含量高于正常组织中的含量。此外，RD 在酸性刺激下对切除的小鼠胃和活体小鼠进行成像时效果良好。这些数据证明了 RD 在监测复杂生物系统 pH 值方面的适用性。(C) 2016 Elsevier Ltd. 保留所有权利。
Intracellular pH is important in regulating various cellular behaviour and pathological conditions. We synthesised a rhodamine-based lysosomal pH probe (hereafter referred to as RD) with a pKa of 4.10. The probe exhibited favourable "on-off' reversibility between pH 3.25 and 7.20 for at least five cycles. RD can be employed to distinguish cancer cells from normal cells on the basis of different fluorescence response. RD performed well in the detection of lysosomal pH induced by artesunate, chloroquine and dexamethasone in MCF-7 cells. The intense fluorescence of RD in ovarian tumour indicated the higher acidity in tumour tissues than that in normal tissues. Furthermore, RD worked well in the imaging of excised mouse stomach and living mice under acidic stimulation. These data demonstrate the applicability of RD in monitoring pH in complex biological systems.(C) 2016 Elsevier Ltd. All rights reserved.