Cutaneous Malassezia flora in atopic dermatitis differs between adults and children
Cutaneous Malassezia flora in atopic dermatitis differs between adults and children
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DOI：
10.1111/j.1365-2133.2007.08193.x
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发表时间：
2007-12-01
影响因子：
10.3
通讯作者：
Muto, M.
中科院分区：
文献类型：
--
作者：
Takahata, Y.;Sugita, T.;Muto, M.
当成人 AD 对抗炎治疗的反应较差时，马拉色菌被怀疑与特应性皮炎 (AD) 皮肤病变的发展有关。然而，尚未对成人和儿童AD的马拉色菌菌群进行比较分析。为比较成人和儿童AD的皮肤马拉色菌菌群，选取58例AD患者头颈部皮损（男28例，女30例；儿童31例，成人27例）鳞片样本，直接提取样本中的真菌DNA。使用基于聚合酶链反应的不依赖于培养物的方法对马拉色菌物种的数量和特性进行了高精度分析。还检测了体内抗马拉色菌IgE抗体水平。AD儿童中以马拉色菌为主，成人中以限制马拉色菌和球形马拉色菌为主。与儿童相比，成人血清中对球形马拉色菌和限制马拉色菌的抗马拉色菌特异性 IgE 反应的敏感性增加。两个年龄组之间皮肤马拉色菌菌群存在显着差异。
Malassezia species are suspected to be involved in the development of skin lesions in atopic dermatitis (AD) when the response of adult AD to anti-inflammatory treatments is poor. However, a comparative analysis of Malassezia flora between adults and children with AD has not been performed.To compare the cutaneous Malassezia flora between adults and children with AD.Scale samples were collected from skin lesions of 58 patients with AD in the head and neck regions (28 males and 30 females; 31 children and 27 adults), and fungal DNA was extracted from the samples directly. The number and identities of the Malassezia species were analysed with high accuracy using a polymerase chain reaction-based culture-independent method. The in vivo level of anti-Malassezia IgE antibody was also assayed.Malassezia restricta was the predominant species in the children with AD, while both M. restricta and M. globosa predominated in the adults. The adults showed increased sensitization in terms of anti-Malassezia-specific IgE responses in the sera to both M. globosa and M. restricta in comparison with the children.The cutaneous Malassezia flora differs significantly between the two age groups.