Hantavirus and arenavirus antibody prevalence in rodents and humans in Trentino, Northern Italy
Hantavirus and arenavirus antibody prevalence in rodents and humans in Trentino, Northern Italy
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DOI：
10.1017/s0950268805005431
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发表时间：
2006-08-01
影响因子：
4.2
通讯作者：
Henttonen, H.
中科院分区：
文献类型：
--
作者：
Kallio-Kokko, H.;Laakkonen, J.;Henttonen, H.
采用免疫荧光法（IFA）研究了意大利Trentino地区汉坦病毒和沙粒病毒抗体阳性野生啮齿动物在2000-2003年和2002年长期捕获的2个点和6个点的时空分布。汉坦病毒在库田鼠（229只）和黄斑弓形鼠（1416只）中分别检测到普鲁马拉病毒（PUUV）抗体和多布拉瓦病毒（DOBV）抗体，阳性率分别为0.4%和0.2%。1472只啮齿动物中有82只（5.6%）存在淋巴细胞性脉络丛脑膜炎病毒（LCMV）抗体；血清阳性率分别为黄斑蠓（1181例）、小光斑蠓（276例）和小仓鼠（7例），分别为6.1%、3.3%和14.3%。在IFA研究的488份林业工人血清样本中，12份LCMV-IgG阳性（2.5%），1份DOBV-IgG阳性（0.2%），但后者无法通过中和试验证实dobv特异性。我们的研究结果表明，DOBV在特伦蒂诺市分布广泛，但患病率较低，并且表明沙粒病毒抗体在除家鼠外的其他几种啮齿动物中也很常见。
The spatial and temporal distribution of hantavirus and arenavirus antibody-positive wild rodents in Trentino, Italy, was studied using immunofluorescence assays (IFA) in two long-term sites trapped in 2000-2003, and six other sites trapped in 2002. The overall hantavirus, seroprevalence in the bank voles, Clethrionomys glareolus (n = 229) screened for Puumala virus (PUUV) antibodies was 0.4 %, and that for Apodemus flavicollis mice (n = 1416) screened for Dobrava virus (DOBV) antibodies was 0.2 %. Antibodies against lymphocytic choriomeningitis virus (LCMV) were found in 82 (5.6 %) of the 1472 tested rodents; the seroprevalence being 6.1 % in A. flavicollis (n = 1181), 3.3 % in C. glareolus (n = 276), and 14.3 % in Microtus arvalis (n = 7). Of the serum samples of 488 forestry workers studied by IFA, 12 were LCMV-IgG positive (2.5 %) and one DOBV-IgG positive (0.2 %), however, the latter could not be confirmed DOBV-specific with a neutralization assay. Our results show a widespread distribution but low prevalence of DOBV in Trentino, and demonstrate that the arenavirus antibodies are a common finding in several other rodent species besides the house mouse.