Differential Contribution of Subunit Interfaces to α9α10 Nicotinic Acetylcholine Receptor Function.
Differential Contribution of Subunit Interfaces to α9α10 Nicotinic Acetylcholine Receptor Function.
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DOI：
10.1124/mol.116.107482
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发表时间：
2017-03
影响因子：
3.6
通讯作者：
Elgoyhen AB
中科院分区：
文献类型：
--
作者：
Boffi JC;Marcovich I;Gill-Thind JK;Corradi J;Collins T;Lipovsek MM;Moglie M;Plazas PV;Craig PO;Millar NS;Bouzat C;Elgoyhen AB
Nicotinic acetylcholine receptors can be assembled from either homomeric or heteromeric pentameric subunit combinations. At the interface of the extracellular domains of adjacent subunits lies the acetylcholine binding site, composed of a principal component provided by one subunit and a complementary component of the adjacent subunit. Compared with neuronal nicotinic acetylcholine cholinergic receptors (nAChRs) assembled from α and β subunits, the α9α10 receptor is an atypical member of the family. It is a heteromeric receptor composed only of α subunits. Whereas mammalian α9 subunits can form functional homomeric α9 receptors, α10 subunits do not generate functional channels when expressed heterologously. Hence, it has been proposed that α10 might serve as a structural subunit, much like a β subunit of heteromeric nAChRs, providing only complementary components to the agonist binding site. Here, we have made use of site-directed mutagenesis to examine the contribution of subunit interface domains to α9α10 receptors by a combination of electrophysiological and radioligand binding studies. Characterization of receptors containing Y190T mutations revealed unexpectedly that both α9 and α10 subunits equally contribute to the principal components of the α9α10 nAChR. In addition, we have shown that the introduction of a W55T mutation impairs receptor binding and function in the rat α9 subunit but not in the α10 subunit, indicating that the contribution of α9 and α10 subunits to complementary components of the ligand-binding site is nonequivalent. We conclude that this asymmetry, which is supported by molecular docking studies, results from adaptive amino acid changes acquired only during the evolution of mammalian α10 subunits.
