Human natural killer cells induce morphologic changes in porcine endothelial cell monolayers.
Human natural killer cells induce morphologic changes in porcine endothelial cell monolayers.
复制标题
人类自然杀伤细胞诱导猪内皮细胞单层的形态变化。
DOI：
10.1097/00007890-199601150-00032
复制
发表时间：
1996
期刊：
影响因子：
6.2
通讯作者：
Dawson,JR
中科院分区：
文献类型：
--
作者：
Malyguine,AM;Saadi,S;Platt,JL;Dawson,JR
在这项研究中，我们研究了自然杀伤（NK）细胞对猪主动脉内皮细胞（PAEC）单层的早期体外效应。在PAEC单层上孵育含有约70% CD 56+细胞的效应细胞导致PAEC单层形态的时间依赖性变化。只要20分钟的孵育导致PAEC形状的变化和细胞之间的间隙的外观。这些影响已被观察到长达6小时，但不是之前20分钟或6小时后。当使用NK-耗尽效应细胞，没有观察到形态学变化与相同的效应细胞耗尽前相比，如果CD 56+细胞被添加回来，效果与那些非耗尽效应细胞。在外周血淋巴细胞与PAEC单层孵育1-6小时期间，未检测到NK细胞介导的细胞溶解活性。这些数据表明NK细胞可能参与导致异种移植排斥的内皮细胞变化。
In this study, we have investigated the early in vitro effects of natural killer (NK) cells on porcine aortic endothelial cell (PAEC) monolayers. Incubation of effector cells containing about 70% CD56+ cells on PAEC monolayers led to time-dependent changes in PAEC monolayer morphology. As little as 20 min of incubation resulted in changes in PAEC shape and in the appearance of gaps between the cells. These effects have been observed for up to 6 hr, but not before 20 min or after 6 hr. When NK-depleted effector cells were used, no morphological changes were observed in comparison with the same effectors before depletion; if CD56+ cells were added back, the effects were comparable with those on nondepleted effector cells. There was no detectable NK cell-mediated cytolytic activity during the 1-6 hr of incubation of peripheral blood lymphocytes with PAEC monolayers. These data indicate that NK cells may participate in endothelial cell changes leading to xenograft rejection.